{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T07:13:54Z","timestamp":1788246834173,"version":"build-2803163510"},"reference-count":17,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["FEBS Open Bio"],"abstract":"<jats:p>The ATP\u2010binding cassette E1 (ABCE1) protein is an essential factor for ribosome recycling, splitting post\u2010termination ribosomes into subunits for subsequent rounds of translation. Despite high conservation, human ABCE1 (hABCE1) fails to functionally complement the depletion of its essential yeast homolog, Rli1. In this study, we leveraged this species\u2010specificity to dissect the functional architecture of ABCE1. Through analysis of yeast\u2013human chimeric proteins, we identified the N\u2010terminal nucleotide\u2010binding domain (NBD1) as the primary determinant of this incompatibility. To further investigate, we isolated multiple hABCE1 point mutants (revertants) that successfully restored yeast growth. We then developed a novel dual\u2010luciferase reporter assay to quantify aberrant translation reinitiation in the 3\u2032UTR, an event recognized as a direct consequence of ABCE1 deficiency. Notably, while the revertant mutants rescued yeast viability, they failed to suppress aberrant reinitiation, exhibiting levels equivalent to the nonfunctional hABCE1. This genetic uncoupling of viability from the suppression of reinitiation suggests that the canonical ribosome recycling function required for cell growth and the role in preventing aberrant reinitiation have distinct functional thresholds or are genetically separable aspects of ABCE1 activity.<\/jats:p>","DOI":"10.1002\/2211-5463.70337","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T06:27:54Z","timestamp":1788244074000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genetic dissection of human\n                    <scp>ABCE1<\/scp>\n                    in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1788-8207","authenticated-orcid":false,"given":"Eriko","family":"Nakata","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Genetics, Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences The\u00a0University of Tokyo  Kashiwa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Genetics, Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences The\u00a0University of Tokyo  Kashiwa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1020-7884","authenticated-orcid":false,"given":"Kei","family":"Endo","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Genetics, Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences The\u00a0University of Tokyo  Kashiwa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2042-405X","authenticated-orcid":false,"given":"Koichi","family":"Ito","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Genetics, Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences The\u00a0University of Tokyo  Kashiwa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.molcel.2009.12.034","article-title":"The role of ABCE1 in eukaryotic posttermination ribosomal recycling","volume":"37","author":"Pisarev AV","year":"2010","journal-title":"Mol Cell"},{"key":"e_1_2_10_3_1","doi-asserted-by":"crossref","first-page":"E1392","DOI":"10.1073\/pnas.1113956108","article-title":"Kinetic analysis reveals the ordered coupling of translation termination and ribosome recycling in yeast","volume":"108","author":"Shoemaker CJ","year":"2011","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/B978-0-12-386497-0.00002-5","article-title":"Termination and post\u2010termination events in eukaryotic translation","volume":"86","author":"Jackson RJ","year":"2012","journal-title":"Adv Protein Chem Struct Biol"},{"key":"e_1_2_10_5_1","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1016\/j.cell.2015.07.041","article-title":"Rli1\/ABCE1 recycles terminating ribosomes and controls translation reinitiation in 3'UTRs in vivo","volume":"162","author":"Young DJ","year":"2015","journal-title":"Cell"},{"key":"e_1_2_10_6_1","first-page":"722","article-title":"Ribosome profiling: new views of translation, from single codons to genome scale","volume":"17","author":"Ingolia NT","year":"2016","journal-title":"Nat Rev Genet"},{"key":"e_1_2_10_7_1","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1038\/nrg2142","article-title":"Translation matters: protein synthesis defects in inherited disease","volume":"8","author":"Scheper GC","year":"2007","journal-title":"Nat Rev Genet"},{"key":"e_1_2_10_8_1","doi-asserted-by":"crossref","first-page":"7820","DOI":"10.1093\/nar\/gky619","article-title":"The ribosomal stalk protein is crucial for the action of the conserved ATPase ABCE1","volume":"46","author":"Imai H","year":"2018","journal-title":"Nucleic Acids Res"},{"key":"e_1_2_10_9_1","doi-asserted-by":"crossref","DOI":"10.15252\/embj.2020105179","article-title":"A structural inventory of native ribosomal ABCE1\u201043S pre\u2010initiation complexes","volume":"40","author":"Kratzat H","year":"2021","journal-title":"EMBO J"},{"key":"e_1_2_10_10_1","doi-asserted-by":"crossref","first-page":"1782","DOI":"10.1002\/2211-5463.13463","article-title":"Human ABCE1 exhibits temperature\u2010dependent heterologous co\u2010functionality in S. 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