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CECs are initially cultured in floating conditions as neurospheres and then expanded in monolayer cultures. RPCs are cultured in floating conditions. Detailed protocols for retinal differentiation, as well as exogenous gene expression using lentivirus are also described. <jats:italic>Curr. Protoc. Stem Cell Biol<\/jats:italic>. 19:1H.4.1\u20101H.4.15. \u00a9 2011 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/9780470151808.sc01h04s19","type":"journal-article","created":{"date-parts":[[2011,12,2]],"date-time":"2011-12-02T22:26:09Z","timestamp":1322864769000},"source":"Crossref","is-referenced-by-count":2,"title":["Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells"],"prefix":"10.1002","volume":"19","author":[{"given":"Sara","family":"Gualdoni","sequence":"first","affiliation":[{"name":"Developmental Biology Unit, UCL Institute of Child Health, University College London  London United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Baron","sequence":"additional","affiliation":[{"name":"Developmental Biology Unit, UCL Institute of Child Health, University College London  London United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rn","family":"Lakowski","sequence":"additional","affiliation":[{"name":"Developmental Biology Unit, UCL Institute of Child Health, University College London  London United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Decembrini","sequence":"additional","affiliation":[{"name":"Developmental Biology Unit, UCL Institute of Child Health, University College London  London United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel A.","family":"Pearson","sequence":"additional","affiliation":[{"name":"Department of Genetics, UCL Institute of Ophthalmology, University College London  London United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin R.","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Genetics, UCL Institute of Ophthalmology, University College London  London United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane C.","family":"Sowden","sequence":"additional","affiliation":[{"name":"Developmental Biology Unit, UCL Institute of Child Health, University College London  London United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.2473"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.04-0697"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0901596106"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0401596101"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.visres.2004.12.017"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.08-3091"},{"key":"e_1_2_9_8_1","first-page":"1842","article-title":"Fibroblast growth factor and epidermal growth factor differently affect differentiation of murine retinal stem cells in vitro","volume":"13","author":"Giordano F.","year":"2007","journal-title":"Mol. 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