{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T22:29:48Z","timestamp":1792708188104,"version":"4.5.0"},"reference-count":15,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,11,6]],"date-time":"2015-11-06T00:00:00Z","timestamp":1446768000000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Stem Cell Biology"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To develop therapeutic strategies for the regeneration of lost heart muscle after myocardial infarction (MI), a source of functional new muscle cells and associated coronary vessels must be identified. The epicardium is a source of several cardiovascular cell types during heart development and is widely regarded as a resident progenitor population, which becomes dormant during adulthood. In adult mice, MI induces epicardial reactivation characterized by an upregulation of fetal genes and subsequent epicardium derived cell (EPDC) proliferation, migration, and differentiation. Determining whether the epicardium can be therapeutically targeted following cardiovascular disease requires an in vitro system for the study of adult human EPDCs (hEPDCs). This protocol describes techniques to establish and maintain human epicardium explant cultures from patient\u2010derived right atrial appendage biopsies and documents methods to probe the resultant outgrowth of hEPDCs. The model facilitates a high\u2010throughput approach to either genetic or chemical phenotypic screening for drug\u2010like modifiers of hEPDC activation and potential cell fate. \u00a9 2015 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/9780470151808.sc02c05s35","type":"journal-article","created":{"date-parts":[[2015,11,4]],"date-time":"2015-11-04T15:18:45Z","timestamp":1446650325000},"source":"Crossref","is-referenced-by-count":10,"title":["The Derivation of Primary Human Epicardium\u2010Derived Cells"],"prefix":"10.1002","volume":"35","author":[{"given":"Caitlin","family":"Clunie\u2010O'Connor","sequence":"first","affiliation":[{"name":"Department of Physiology, Anatomy and Genetics, University of Oxford  Oxford United Kingdom"},{"name":"Department of Chemistry, Chemistry Research Laboratory  Oxford United Kingdom"},{"name":"These authors contributed equally to this study"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anke M.","family":"Smits","sequence":"additional","affiliation":[{"name":"Department of Molecular Cell Biology, Leiden University Medical Centre  Leiden The Netherlands"},{"name":"These authors contributed equally to this study"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charalambos","family":"Antoniades","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine, University of Oxford, John Radcliffe Hospital  Oxford United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela J.","family":"Russell","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Chemistry Research Laboratory  Oxford United Kingdom"},{"name":"Department of Pharmacology, University of Oxford  Oxford United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek M.","family":"Yellon","sequence":"additional","affiliation":[{"name":"The Hatter Cardiovascular Institute, University College  London United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010Jos\u00e9","family":"Goumans","sequence":"additional","affiliation":[{"name":"Department of Molecular Cell Biology, Leiden University Medical Centre  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul R.","family":"Riley","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Genetics, University of Oxford  Oxford United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,11,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395\u2010011\u20100181\u20100"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2013.10.005"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06969"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0796"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.107.150755"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.02.022"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470151808.sc02c02s8"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10188"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2006\u20100366"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0044692"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.a.10129"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018\u2010007\u20106522\u20103"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1417232112"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07060"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI45529"}],"container-title":["Current Protocols in Stem Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2F9780470151808.sc02c05s35","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/9780470151808.sc02c05s35","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/9780470151808.sc02c05s35","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/9780470151808.sc02c05s35","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T01:00:09Z","timestamp":1692579609000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/9780470151808.sc02c05s35"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11]]},"references-count":15,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,11]]}},"alternative-id":["10.1002\/9780470151808.sc02c05s35"],"URL":"https:\/\/doi.org\/10.1002\/9780470151808.sc02c05s35","archive":["Portico"],"relation":{},"ISSN":["1941-7322","1938-8969"],"issn-type":[{"value":"1941-7322","type":"print"},{"value":"1938-8969","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,11]]}}}