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After library selection, changes in the frequency of each insertion mutant are determined by sequencing flanking regions en masse. These changes are used to calculate each mutant's fitness. The method was originally developed for the Gram\u2010positive bacterium <jats:italic>Streptococcus pneumoniae<\/jats:italic>, a causative agent of pneumonia and meningitis, but has now been applied to several different microbial species.<\/jats:p>","DOI":"10.1002\/9780471729259.mc01e03s36","type":"journal-article","created":{"date-parts":[[2015,2,2]],"date-time":"2015-02-02T11:58:07Z","timestamp":1422878287000},"source":"Crossref","is-referenced-by-count":49,"title":["Genome\u2010Wide Fitness and Genetic Interactions Determined by Tn\u2010seq, a High\u2010Throughput Massively Parallel Sequencing Method for Microorganisms"],"prefix":"10.1002","volume":"36","author":[{"given":"Tim","family":"van Opijnen","sequence":"first","affiliation":[{"name":"Department of Biology Boston College Chestnut Hill Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Lazinski","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Microbiology School of Medicine Tufts University Howard Hughes Medical Institute Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Camilli","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Microbiology School of Medicine Tufts University Howard Hughes Medical Institute Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,2]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.15.8927"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-9525(92)90263-4"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-321-9_7"},{"key":"e_1_2_11_5_1","first-page":"103","article-title":"Damage recovery pathways in Saccharomyces cerevisiae revealed by ge\u2010nomic phenotyping and interactome mapping","volume":"1","author":"Begley T.J.","year":"2002","journal-title":"Mol. 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