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As an example, the viral RNA genome of the 1918 pandemic influenza A virus was previously determined in a 9\u2010year effort by overlapping RT\u2010PCR from post\u2010mortem samples. Using the protocols described here, the full genome of the 1918 virus was determined at high coverage in one high\u2010throughput sequencing run of a cDNA library derived from total RNA of a 1918 FFPE sample after duplex\u2010specific nuclease treatments. This basic methodological approach should assist in the analysis of FFPE tissue samples isolated over the past century from a variety of infectious diseases. \u00a9 2015 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/9780471729259.mc01e08s37","type":"journal-article","created":{"date-parts":[[2015,5,1]],"date-time":"2015-05-01T10:44:38Z","timestamp":1430477078000},"source":"Crossref","is-referenced-by-count":6,"title":["Isolating Viral and Host RNA Sequences from Archival Material and Production of cDNA Libraries for High\u2010Throughput DNA Sequencing"],"prefix":"10.1002","volume":"37","author":[{"given":"Yongli","family":"Xiao","sequence":"first","affiliation":[{"name":"Viral Pathogenesis and Evolution Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health  Bethesda 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