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Two issues affecting the culture and storage of isolates of this slow\u2010growing bacterium are emphasized: contamination of cultures and outgrowth of attenuated variants lacking a complete lipopolysaccharide. Laboratories planning to work with <jats:named-content content-type=\"genus-species\"><jats:italic>B. abortus<\/jats:italic><\/jats:named-content> should be aware that Biosafety Level 3 facilities are required. Furthermore, this organism is classified in the U.S. as a Select Agent, which restricts its use to laboratories registered with the U.S. government's Select Agent programs.<\/jats:p>","DOI":"10.1002\/9780471729259.mc03b01s00","type":"journal-article","created":{"date-parts":[[2005,10,25]],"date-time":"2005-10-25T14:20:34Z","timestamp":1130250034000},"source":"Crossref","is-referenced-by-count":1,"title":["Laboratory Maintenance of <i>Brucella abortus<\/i>"],"prefix":"10.1002","volume":"00","author":[{"given":"Yao\u2010Hui","family":"Sun","sequence":"first","affiliation":[{"name":"University of California at Davis  Davis California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas B.","family":"den Hartigh","sequence":"additional","affiliation":[{"name":"University of California at Davis  Davis California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ren\u00e9e M.","family":"Tsolis","sequence":"additional","affiliation":[{"name":"University of California at Davis  Davis California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas A.","family":"Ficht","sequence":"additional","affiliation":[{"name":"Texas A&amp;M University  College Station Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,10]]},"reference":[{"key":"e_1_2_8_2_1_1","volume-title":"Laboratory Techniques in Brucellosis","author":"Alton G.G.","year":"1975"},{"key":"e_1_2_8_2_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.5.2005-2006.2000"},{"key":"e_1_2_8_2_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00210-9"},{"key":"e_1_2_8_3_2_1","unstructured":"Altonet al. 1975. 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