{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T17:08:25Z","timestamp":1790960905171,"version":"4.1.0"},"reference-count":15,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,2,1]],"date-time":"2008-02-01T00:00:00Z","timestamp":1201824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2008,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:named-content content-type=\"genus-species\"><jats:italic>Neisseria gonorrhoeae<\/jats:italic><\/jats:named-content> is a human pathogen of mucosal surfaces, thus laboratory manipulations must include appropriate safety measures. The growth requirements and behavior of the gonococcus are significantly different from many bacteria, necessitating modifications of common laboratory techniques. A fastidious organism, <jats:named-content content-type=\"genus-species\"><jats:italic>N. gonorrhoeae<\/jats:italic><\/jats:named-content> requires enriched media in a CO<jats:sub>2<\/jats:sub> atmosphere at 35\u00b0 to 37\u00b0C for growth. In addition, <jats:named-content content-type=\"genus-species\"><jats:italic>N. gonorrhoeae<\/jats:italic><\/jats:named-content> expresses potent autolysins whose activity increases following glucose depletion during stationary phase, leading to cell death. Long believed to be an obligate aerobe, the gonococcus is capable of anaerobic growth when provided with a suitable electron acceptor. This unit provides information for both aerobic and anaerobic growth, basic long\u2010term and daily maintenance of gonococcal cultures, as well as safety considerations for laboratory studies. <jats:italic>Curr. Protoc. Microbiol<\/jats:italic>. 8:4A.1.1\u20104A.1.26. \u00a9 2008 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/9780471729259.mc04a01s8","type":"journal-article","created":{"date-parts":[[2008,2,22]],"date-time":"2008-02-22T15:49:23Z","timestamp":1203695363000},"source":"Crossref","is-referenced-by-count":34,"title":["Laboratory Maintenance of <i>Neisseria gonorrhoeae<\/i>"],"prefix":"10.1002","volume":"8","author":[{"given":"Janice M.","family":"Spence","sequence":"first","affiliation":[{"name":"University of Rochester  Rochester New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lori","family":"Wright","sequence":"additional","affiliation":[{"name":"University of Rochester  Rochester New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia L.","family":"Clark","sequence":"additional","affiliation":[{"name":"University of Rochester  Rochester New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,2]]},"reference":[{"key":"e_1_2_12_2_1_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02520.x"},{"key":"e_1_2_12_2_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.128.1.192-201.1976"},{"key":"e_1_2_12_2_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.85.6.1274-1279.1963"},{"key":"e_1_2_12_2_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.96.3.596-605.1968"},{"key":"e_1_2_12_2_5_1","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1128\/iai.46.1.176-181.1984","article-title":"Anaerobic growth of Neisseria gonorrhoeae coupled to nitrite reduction","volume":"46","author":"Knapp J.S.","year":"1984","journal-title":"Infect. 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