{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T21:20:44Z","timestamp":1792099244066,"version":"4.4.0"},"reference-count":57,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,11,1]],"date-time":"2011-11-01T00:00:00Z","timestamp":1320105600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The sexually transmitted pathogen,\n                    <jats:named-content content-type=\"genus-species\">\n                      <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n                    <\/jats:named-content>\n                    , undergoes natural transformation at high frequency. This property has led to the rapid dissemination of antibiotic resistance markers and to the panmictic structure of the gonococcal population. However, high\u2010frequency transformation also makes\n                    <jats:named-content content-type=\"genus-species\">\n                      <jats:italic>N. gonorrhoeae<\/jats:italic>\n                    <\/jats:named-content>\n                    one of the easiest bacterial species to manipulate genetically in the laboratory. Techniques have been developed that result in transformation frequencies &gt;50%, allowing the identification of mutants by screening and without selection. Constructs have been created to take advantage of this high\u2010frequency transformation, facilitating genetic mutation, complementation, and heterologous gene expression. Techniques are described for genetic manipulation of\n                    <jats:named-content content-type=\"genus-species\">\n                      <jats:italic>N. gonorrhoeae<\/jats:italic>\n                    <\/jats:named-content>\n                    , as well as for growth of this fastidious organism.\n                    <jats:italic>Curr. Protoc. Microbiol<\/jats:italic>\n                    . 23:4A.2.1\u20104A.2.24. \u00a9 2011 by John Wiley &amp; Sons, Inc.\n                  <\/jats:p>","DOI":"10.1002\/9780471729259.mc04a02s23","type":"journal-article","created":{"date-parts":[[2011,11,1]],"date-time":"2011-11-01T08:28:47Z","timestamp":1320136127000},"source":"Crossref","is-referenced-by-count":99,"title":["Genetic Manipulation of\n                    <i>Neisseria gonorrhoeae<\/i>"],"prefix":"10.1002","volume":"23","author":[{"given":"Joseph P.","family":"Dillard","sequence":"first","affiliation":[{"name":"Medical Microbiology and Immunology, University of Wisconsin\u2010Madison  Madison Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,11]]},"reference":[{"key":"e_1_2_16_2_1_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01408-06"},{"key":"e_1_2_16_2_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb02081.x"},{"key":"e_1_2_16_2_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(97)00653-7"},{"key":"e_1_2_16_2_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142727.mb0302s13"},{"key":"e_1_2_16_2_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(93)90695-Y"},{"key":"e_1_2_16_2_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(94)00890-5"},{"key":"e_1_2_16_2_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1175653"},{"key":"e_1_2_16_2_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.160.1.288-293.1984"},{"key":"e_1_2_16_2_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro844"},{"key":"e_1_2_16_2_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.6.2752-2757.2002"},{"key":"e_1_2_16_2_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1998.tb12981.x"},{"key":"e_1_2_16_2_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb01084.x"},{"key":"e_1_2_16_2_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.12.3911-3913.1991"},{"key":"e_1_2_16_2_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142727.mb0106s15"},{"key":"e_1_2_16_2_15_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-137-6-1313"},{"key":"e_1_2_16_2_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1996.tb08574.x"},{"key":"e_1_2_16_2_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.18.6982"},{"key":"e_1_2_16_2_18_1","first-page":"509","article-title":"Use of a nonselective transformation technique to construct a multiply restriction\/modification\u2010deficient mutant of Neisseria gonorrhoeae","volume":"251","author":"Gunn J.S.","year":"1996","journal-title":"Mol. 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