{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:35:05Z","timestamp":1787506505036,"version":"build-2736575974"},"reference-count":9,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,11,1]],"date-time":"2009-11-01T00:00:00Z","timestamp":1257033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2009,11]]},"abstract":"Abstract<\/jats:title>\n \n \n Coxiella burnetii<\/jats:italic>\n <\/jats:named-content>\n , an obligate intracellular Gram\u2010negative bacterium, is the agent of Q fever, a self\u2010limited flu\u2010like illness that may also present as chronic endocarditis. The ability to persist in the environment at a low infectious dose in aerosols resulted in the classification of\n \n C. burnetii<\/jats:italic>\n <\/jats:named-content>\n as a BSL\u20103 select agent. Routine propagation of this agent is in embryonated eggs or tissue culture. Purification from host tissues includes multiple differential centrifugations to separate bacteria from host material. Infection of host cells is routinely verified microscopically by using Gimenez stain, fluorescent dyes, or immunofluorescence antibody (IFA) staining. Identification of\n \n C. burnetii<\/jats:italic>\n <\/jats:named-content>\n DNA in host material is measured by PCR. Quantification of purified\n \n C. burnetii<\/jats:italic>\n <\/jats:named-content>\n is accomplished through conversion of optical density to dry weight or, more precisely, by RT\u2010PCR to determine genome equivalents. Serum antibody titer to\n \n C. burnetii<\/jats:italic>\n <\/jats:named-content>\n is determined by microagglutination assay or ELISA.\n Curr. Protoc. Microbiol<\/jats:italic>\n . 15:6C.1.1\u20106C.1.16. \u00a9 2009 by John Wiley & Sons, Inc.\n <\/jats:p>","DOI":"10.1002\/9780471729259.mc06c01s15","type":"journal-article","created":{"date-parts":[[2009,11,5]],"date-time":"2009-11-05T02:21:12Z","timestamp":1257387672000},"source":"Crossref","is-referenced-by-count":26,"title":["Laboratory Maintenance of\n Coxiella burnetii<\/i>"],"prefix":"10.1002","volume":"15","author":[{"given":"James E.","family":"Samuel","sequence":"first","affiliation":[{"name":"Texas A&M Health Science Center College Station Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura R.","family":"Hendrix","sequence":"additional","affiliation":[{"name":"Texas A&M Health Science Center College Station Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,11]]},"reference":[{"key":"e_1_2_18_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.5.1869-1874.2003"},{"key":"e_1_2_18_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.5.3240"},{"key":"e_1_2_18_4_1","first-page":"2857","article-title":"Active transport of proline by Coxiella burnetii","volume":"130","author":"Hendrix L.R.","year":"1984","journal-title":"J. Gen. Microbiol"},{"key":"e_1_2_18_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-137-2-269"},{"key":"e_1_2_18_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00918345"},{"key":"e_1_2_18_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.41.2.488-493.1983"},{"key":"e_1_2_18_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.49.3.775-779.1985"},{"key":"e_1_2_18_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.11.6026-6033.1999"},{"key":"e_1_2_18_10_1","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1128\/iai.32.2.840-851.1981","article-title":"Immunological and biological characterization of Coxiella burnetii, phase I and phase II, separated from host components","volume":"32","author":"Williams J.C.","year":"1981","journal-title":"Infect. Immun"}],"container-title":["Current Protocols in Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2F9780471729259.mc06c01s15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/9780471729259.mc06c01s15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/9780471729259.mc06c01s15","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/9780471729259.mc06c01s15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T15:50:18Z","timestamp":1694188218000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/9780471729259.mc06c01s15"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,11]]},"references-count":9,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2009,11]]}},"alternative-id":["10.1002\/9780471729259.mc06c01s15"],"URL":"https:\/\/doi.org\/10.1002\/9780471729259.mc06c01s15","archive":["Portico"],"relation":{},"ISSN":["1934-8525","1934-8533"],"issn-type":[{"value":"1934-8525","type":"print"},{"value":"1934-8533","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,11]]}}}