{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T02:08:45Z","timestamp":1793930925442,"version":"4.8.0"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,2,6]],"date-time":"2014-02-06T00:00:00Z","timestamp":1391644800000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n<jats:italic>Staphylococcus aureus<\/jats:italic> is a facultative anaerobic Gram\u2010positive coccus and a member of the normal skin flora, as well as that of the nasal passages of humans. However, <jats:italic>S. aureus<\/jats:italic> can also gain entry into the host and cause life\u2010threatening infections or persist as disease foci that develop into suppurative abscesses. While genetically tractable, the manipulation of <jats:italic>S. aureus<\/jats:italic> remains challenging. This unit describes methods developed in our laboratory for gene disruption by allelic replacement and transposition. We also provide a protocol for bacteriophage\u2010mediated transduction of mutants marked with selectable alleles and describe plasmid utilization for complementation studies. <jats:italic>Curr. Protoc. Microbiol<\/jats:italic>. 32:9C.3.1\u20109C.3.19. \u00a9 2014 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/9780471729259.mc09c03s32","type":"journal-article","created":{"date-parts":[[2014,2,6]],"date-time":"2014-02-06T13:23:46Z","timestamp":1391693026000},"source":"Crossref","is-referenced-by-count":39,"title":["Genetic Manipulation of <i>Staphylococcus aureus<\/i>"],"prefix":"10.1002","volume":"32","author":[{"given":"Olaf","family":"Schneewind","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Chicago  Chicago, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Missiakas","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Chicago  Chicago, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,2,6]]},"reference":[{"key":"e_1_2_11_2_1","volume-title":"Short Protocols in Molecular Biology","author":"Ausubel F.M.","year":"2002"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2005.05.005"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404728101"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(92)90629-X"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0603072103"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0405620102"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.7.3738-3744.1990"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.4.1046-1052.2000"},{"key":"e_1_2_11_10_1","first-page":"432","article-title":"Molecular genetic analysis of staphylococcal virulence","volume":"27","author":"Foster T.J.","year":"1998","journal-title":"Methods Microbiol."},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.62.4.1371-1414.1998"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.mmi_17061143.x"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.143000"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0147-619X(86)90078-8"},{"key":"e_1_2_11_15_1","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1128\/jb.124.2.597-601.1975","article-title":"Recombinant plasmid obtained from two different, compatible staphylococcal plasmids","volume":"124","author":"Iordanescu S.","year":"1975","journal-title":"J. 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