{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T04:28:34Z","timestamp":1769920114613,"version":"3.49.0"},"reference-count":13,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,2,16]],"date-time":"2018-02-16T00:00:00Z","timestamp":1518739200000},"content-version":"vor","delay-in-days":1599,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Streptococcus pyogenes<\/jats:italic> (the Group A Streptococcus, GAS) is a Gram\u2010positive bacterium responsible for a wide spectrum of diseases ranging from mild superficial infections (pharyngitis, impetigo) to severe, often life\u2010threatening invasive diseases (necrotizing fasciitis, streptococcal toxic shock syndrome) in humans. This unit describes molecular techniques for the genetic manipulation of <jats:italic>S. pyogenes<\/jats:italic> with detailed protocols for transformation, gene disruption, allelic exchange, transposon mutagenesis, and genetic complementation. <jats:italic>Curr. Protoc. Microbiol<\/jats:italic>. 30:9D.3.1\u20109D.3.29. \u00a9 2013 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/9780471729259.mc09d03s30","type":"journal-article","created":{"date-parts":[[2013,10,22]],"date-time":"2013-10-22T15:42:14Z","timestamp":1382456534000},"source":"Crossref","is-referenced-by-count":50,"title":["Genetic Manipulation of <i>Streptococcus pyogenes<\/i> (The Group A Streptococcus, GAS)"],"prefix":"10.1002","volume":"30","author":[{"given":"Yoann","family":"Le Breton","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics, Maryland Pathogen Research Institute University of Maryland  College Park Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin S.","family":"McIver","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics, Maryland Pathogen Research Institute University of Maryland  College Park Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,2,16]]},"reference":[{"key":"e_1_2_16_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2009.03.002"},{"key":"e_1_2_16_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(91)04028-M"},{"key":"e_1_2_16_4_1","first-page":"59","volume-title":"Gram\u2010Positive Pathogens","author":"Cho K.H.","year":"2006"},{"key":"e_1_2_16_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.4.1194-1201.1991"},{"key":"e_1_2_16_6_1","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1128\/iai.63.1.345-348.1995","article-title":"Expression of the Arp protein, a member of the M protein family, is not sufficient to inhibit phagocytosis of Streptococcus pyogenes","volume":"63","author":"Husmann L.K.","year":"1995","journal-title":"Infect. 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