{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T10:34:01Z","timestamp":1774780441429,"version":"3.50.1"},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,2,8]],"date-time":"2016-02-08T00:00:00Z","timestamp":1454889600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Nocardia<\/jats:italic>spp. are aerobic, Gram\u2010positive, catalase positive, and non\u2010motile actinomycetes. They are associated with human infections. However, some species produce important natural products, degrade toxic chemicals, and are involved in biotransformation of valuable products. The lack of robust genetic tools has hindered detailed studies and advanced research. This unit describes the major genetic engineering approaches using<jats:italic>Nocardia<\/jats:italic>sp. CS682 as a prototype. These methods will certainly help in understanding the basis of their pathogenicity as well as biosynthetic and biotransforming abilities. It can be expected that knowledge of the biochemistry behind their pathogenicity will be crucial in developing effective treatment strategies. These genetic tools can be utilized to develop rational metabolic engineering approaches for crafting host strains with higher production or biotransformation ability. \u00a9 2016 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/9780471729259.mc10f02s40","type":"journal-article","created":{"date-parts":[[2016,2,9]],"date-time":"2016-02-09T12:50:09Z","timestamp":1455022209000},"source":"Crossref","is-referenced-by-count":12,"title":["Genetic Manipulation of<i>Nocardia<\/i>Species"],"prefix":"10.1002","volume":"40","author":[{"given":"Dipesh","family":"Dhakal","sequence":"first","affiliation":[{"name":"Institute of Biomolecule Reconstruction, Department of BT\u2010Convergent Pharmaceutical Engineering, Sun Moon University, Chungnam Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amit","family":"Kumar Jha","sequence":"additional","affiliation":[{"name":"Institute of Biomolecule Reconstruction, Department of BT\u2010Convergent Pharmaceutical Engineering, Sun Moon University, Chungnam Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anaya","family":"Pokhrel","sequence":"additional","affiliation":[{"name":"Institute of Biomolecule Reconstruction, Department of BT\u2010Convergent Pharmaceutical Engineering, Sun Moon University, Chungnam Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anil","family":"Shrestha","sequence":"additional","affiliation":[{"name":"Institute of Biomolecule Reconstruction, Department of BT\u2010Convergent Pharmaceutical Engineering, Sun Moon University, Chungnam Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae Kyung","family":"Sohng","sequence":"additional","affiliation":[{"name":"Institute of Biomolecule Reconstruction, Department of BT\u2010Convergent Pharmaceutical Engineering, Sun Moon University, Chungnam Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,2,8]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_12_3_1","first-page":"489","article-title":"Bacteremic infection due to Nocardia otitidiscaviarum: Case report and review","volume":"22","author":"Candel F.J.","year":"2005","journal-title":"An. 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