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This unit describes methods for the isolation of subspecies of <jats:named-content content-type=\"genus-species\"><jats:italic>Treponema pallidum<\/jats:italic><\/jats:named-content> and other pathogenic treponemes from clinical specimens, the propagation of these isolates in rabbits, isolation of clonal populations of <jats:named-content content-type=\"genus-species\"><jats:italic>T. pallidum<\/jats:italic><\/jats:named-content>, and techniques for maintenance of frozen stocks of these treponemes. <jats:italic>Curr. Protoc. Microbiol<\/jats:italic>. 7:12A.1.1\u201012A.1.18. \u00a9 2007 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/9780471729259.mc12a01s7","type":"journal-article","created":{"date-parts":[[2007,11,14]],"date-time":"2007-11-14T15:46:49Z","timestamp":1195055209000},"source":"Crossref","is-referenced-by-count":71,"title":["Isolation and Laboratory Maintenance of <i>Treponema pallidum<\/i>"],"prefix":"10.1002","volume":"7","author":[{"given":"Sheila A.","family":"Lukehart","sequence":"first","affiliation":[{"name":"University of Washington  Seattle Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina M.","family":"Marra","sequence":"additional","affiliation":[{"name":"University of Washington  Seattle Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,11]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(01)01513-1"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.2.824-831.2000"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04086.x"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.32.2.908-915.1981"},{"key":"e_1_2_11_6_1","first-page":"474","article-title":"Note sur quelques aspects immunologiques du cynocephale africain","volume":"56","author":"Fribourg\u2010Blanc A.","year":"1963","journal-title":"Bull. 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