{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:37:21Z","timestamp":1792654641674,"version":"4.5.0"},"reference-count":61,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,2,15]],"date-time":"2011-02-15T00:00:00Z","timestamp":1297728000000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2011,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The development of robust genetic tools to manipulate <jats:named-content content-type=\"genus-species\"><jats:italic>Borrelia burgdorferi<\/jats:italic><\/jats:named-content>, the etiologic agent of Lyme disease, now allows investigators to assess the role(s) of individual genes in the context of experimental Lyme borreliosis. This unit is devoted to the description of experimental approaches that are available for the molecular genetic analysis of <jats:named-content content-type=\"genus-species\"><jats:italic>B. burgdorferi<\/jats:italic><\/jats:named-content> with an emphasis on cultivation, electrotransformation, selection of desired mutants, and genetic complementation of acquired mutants. The intent is to provide a consensus protocol that encapsulates the methodologies currently employed by the <jats:named-content content-type=\"genus-species\"><jats:italic>B. burgdorferi<\/jats:italic><\/jats:named-content> research community and describe pertinent issues that must be accounted for when working with these pathogenic spirochetal bacteria. <jats:italic>Curr. Protoc. Microbiol<\/jats:italic>. 20:12C.4.1\u201012C.4.17. \u00a9 2011 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/9780471729259.mc12c04s20","type":"journal-article","created":{"date-parts":[[2011,2,4]],"date-time":"2011-02-04T15:13:36Z","timestamp":1296832416000},"source":"Crossref","is-referenced-by-count":13,"title":["Genetic Transformation of <i>Borrelia burgdorferi<\/i>"],"prefix":"10.1002","volume":"20","author":[{"given":"Jenny A.","family":"Hyde","sequence":"first","affiliation":[{"name":"Texas A&amp;M University Health Science Center  College Station Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric H.","family":"Weening","sequence":"additional","affiliation":[{"name":"University of North Carolina, Chapel Hill, School of Medicine  Chapel Hill North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jon T.","family":"Skare","sequence":"additional","affiliation":[{"name":"Texas A&amp;M University Health Science Center  College Station Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,2,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.012602299"},{"key":"e_1_2_9_3_1","first-page":"521","article-title":"Isolation and cultivation of Lyme disease spirochetes","volume":"57","author":"Barbour A.G.","year":"1984","journal-title":"Yale J. Biol. Med."},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/157.4.842"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/162.1.133"},{"key":"e_1_2_9_6_1","first-page":"359","volume-title":"Animal models of Borreliosis. In Borrelia: Molecular Biology, Host Interaction and Pathogenesis","author":"Barthold S.W.","year":"2010"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04688.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02454-06"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.9.2445-2452.2000"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00993-06"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06204.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7043737"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.11.3561-3569.2004"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.11.6433-6445.2004"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.1.217-227.1997"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01698.x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00324-08"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI39401"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02758.x"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.4.2139-2150.2002"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1159\/000073406"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.22.6723-6727.2003"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/37551"},{"key":"e_1_2_9_24_1","first-page":"103","volume-title":"Borrelia: Molecular Biology, Host Interaction and Pathogenesis","author":"Gherardini F.C.","year":"2010"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05593.x"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.10.5938-5946.2004"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06951.x"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.12.7147-7154.2004"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00318-09"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.1.446-455.2001"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.8.4608-4613.2003"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.9.4798-4804.2002"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05550.x"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000679"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2005.05.013"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)01146-5"},{"key":"e_1_2_9_37_1","volume-title":"Pathobiology of Lyme disease Borrelia. In: Borrelia: Molecular Biology, Host Interaction and Pathogenesis","author":"Norris S.J.","year":"2010"},{"key":"e_1_2_9_38_1","first-page":"279","volume-title":"Tick interactions. In: Borrelia: Molecular Biology, Host Interaction and Pathogenesis","author":"Pal U.","year":"2010"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.5.3016-3020.2006"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.25.3.557-558.1987"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.31.5.1251-1255.1993"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.25.13865"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03452.x"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0502565102"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1086"},{"key":"e_1_2_9_46_1","first-page":"189","volume-title":"Genetic manipulation of Borrelia burgdorferi. In: Borrelia: Molecular Biology, Host Interaction and Pathogenesis","author":"Rosa P.A.","year":"2010"},{"key":"e_1_2_9_47_1","first-page":"253","article-title":"Electrotransformation of the spirochete Borrelia burgdorferi","volume":"47","author":"Samuels D.S.","year":"1995","journal-title":"Methods Mol. Biol."},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.080068797"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(02)01146-0"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.26.5.893-895.1988"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04352.x"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.05042.x"},{"key":"e_1_2_9_53_1","volume-title":"Gene regulation, transcriptomics and proteomics. In: Borrelia: Molecular Biology, Host Interaction and Pathogenesis","author":"Skare J.T.","year":"2010"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198303313081301"},{"key":"e_1_2_9_55_1","doi-asserted-by":"crossref","unstructured":"Steere A.C. Coburn J. andGlickstein L.2004.The emergence of Lyme disease. J. Clin. Invest.113:1093\u20101101.","DOI":"10.1172\/JCI21681"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.18.4850-4855.1998"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02256.x"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.10.3202-3209.2003"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.10.5973-5979.2004"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00690-08"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1834315100"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20031960"}],"container-title":["Current Protocols in Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2F9780471729259.mc12c04s20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/9780471729259.mc12c04s20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/9780471729259.mc12c04s20","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/9780471729259.mc12c04s20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T01:19:23Z","timestamp":1693617563000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/9780471729259.mc12c04s20"}},"subtitle":["Spirochetes"],"short-title":[],"issued":{"date-parts":[[2011,2]]},"references-count":61,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,2]]}},"alternative-id":["10.1002\/9780471729259.mc12c04s20"],"URL":"https:\/\/doi.org\/10.1002\/9780471729259.mc12c04s20","archive":["Portico"],"relation":{},"ISSN":["1934-8525","1934-8533"],"issn-type":[{"value":"1934-8525","type":"print"},{"value":"1934-8533","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,2]]}}}