{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:02:17Z","timestamp":1791176537782,"version":"4.1.0"},"reference-count":14,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,10,1]],"date-time":"2006-10-01T00:00:00Z","timestamp":1159660800000},"content-version":"vor","delay-in-days":242,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Analysis of <jats:named-content content-type=\"genus-species\"><jats:italic>Leptospira<\/jats:italic><\/jats:named-content> requires use of specialized media for growth, maintenance, and storage of viable bacteria that can be used in experimental protocols in a predictable manner. However, pathogenic <jats:named-content content-type=\"genus-species\"><jats:italic>Leptospira<\/jats:italic><\/jats:named-content> are fastidious bacteria with unusual nutritional requirements. These problems make primary isolation, routine propagation, and storage of <jats:named-content content-type=\"genus-species\"><jats:italic>Leptospira<\/jats:italic><\/jats:named-content> difficult. Defined and complex media are available for routine growth of pathogenic <jats:named-content content-type=\"genus-species\"><jats:italic>Leptospira<\/jats:italic><\/jats:named-content>, and each medium has unique characteristics that favor the growth or isolation of some strains, while not supporting the growth of others. This unit will describe the preparation of <jats:named-content content-type=\"genus-species\"><jats:italic>Leptospira<\/jats:italic><\/jats:named-content> media and methods for isolation and storage of these bacteria.<\/jats:p>","DOI":"10.1002\/9780471729259.mc12e01s00","type":"journal-article","created":{"date-parts":[[2005,10,25]],"date-time":"2005-10-25T14:20:34Z","timestamp":1130250034000},"source":"Crossref","is-referenced-by-count":35,"title":["Laboratory Maintenance of Pathogenic <i>Leptospira<\/i>"],"prefix":"10.1002","volume":"00","author":[{"given":"Richard L.","family":"Zuerner","sequence":"first","affiliation":[{"name":"National Animal Disease Center USDA, ARS, MWA  Ames Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,10]]},"reference":[{"key":"e_1_2_9_2_1_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.97.3.992-1000.1969"},{"key":"e_1_2_9_2_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-49-2-839"},{"key":"e_1_2_9_2_3_1","first-page":"45","article-title":"Nutrition of Leptospira\n                  pomona and growth of 13 other serotypes: Fractionation of oleic albumin complex and medium bovine albumin and polysorbate 80","volume":"26","author":"Ellinghausen H.C.","year":"1965","journal-title":"Am. 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Seeabove."}],"container-title":["Current Protocols in Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/9780471729259.mc12e01s00","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/9780471729259.mc12e01s00","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/9780471729259.mc12e01s00","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T16:54:11Z","timestamp":1692982451000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/9780471729259.mc12e01s00"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,2]]},"references-count":14,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2006,2]]}},"alternative-id":["10.1002\/9780471729259.mc12e01s00"],"URL":"https:\/\/doi.org\/10.1002\/9780471729259.mc12e01s00","archive":["Portico"],"relation":{},"ISSN":["1934-8525","1934-8533"],"issn-type":[{"value":"1934-8525","type":"print"},{"value":"1934-8533","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,2]]}}}