{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T13:59:44Z","timestamp":1792418384451,"version":"4.5.0"},"reference-count":10,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,2,1]],"date-time":"2008-02-01T00:00:00Z","timestamp":1201824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2008,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Lymphocytic choriomeningitis virus (LCMV) is an enveloped, ambisense RNA virus and the prototypic virus of the arenavirus group. It can cause viral meningitis and other ailments in humans, but its natural host is the mouse. The LCMV\/mouse model has been useful for examining mechanisms of viral persistence and the basic concepts of virus\u2010induced immunity and immunopathology. This unit discusses strain differences and biosafety containment issues for LCMV. Recommendations are made for techniques for propagating LCMV to high titers to quantify it by plaque assay and PCR techniques and to preserve its infectivity by appropriate storage.\n                    <jats:italic>Curr. Protoc. Microbiol<\/jats:italic>\n                    . 8:15A.1.1\u201015A.1.11. \u00a9 2008 by John Wiley &amp; Sons, Inc.\n                  <\/jats:p>","DOI":"10.1002\/9780471729259.mc15a01s8","type":"journal-article","created":{"date-parts":[[2008,2,22]],"date-time":"2008-02-22T10:49:23Z","timestamp":1203677363000},"source":"Crossref","is-referenced-by-count":65,"title":["Lymphocytic Choriomeningitis Virus (LCMV): Propagation, Quantitation, and Storage"],"prefix":"10.1002","volume":"8","author":[{"given":"Raymond M.","family":"Welsh","sequence":"first","affiliation":[{"name":"University of Massachusetts Medical School  Worcester Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mina O.","family":"Seedhom","sequence":"additional","affiliation":[{"name":"University of Massachusetts Medical School  Worcester Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5396.2079"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.040558797"},{"key":"e_1_2_9_4_1","first-page":"175","article-title":"Reverse genetics of arenaviruses","volume":"262","author":"Lee K.J.","year":"2002","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1995.1314"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2004.06.009"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/362758a0"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.6.3454"},{"key":"e_1_2_9_9_1","first-page":"289","volume-title":"Effects of Microbes on the Immune System","author":"Welsh R.M.","year":"2000"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.145.6.1449"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.12.2205"}],"container-title":["Current Protocols in Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2F9780471729259.mc15a01s8","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/9780471729259.mc15a01s8","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/9780471729259.mc15a01s8","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/9780471729259.mc15a01s8","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T14:25:54Z","timestamp":1693751154000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/9780471729259.mc15a01s8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,2]]},"references-count":10,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,2]]}},"alternative-id":["10.1002\/9780471729259.mc15a01s8"],"URL":"https:\/\/doi.org\/10.1002\/9780471729259.mc15a01s8","archive":["Portico"],"relation":{},"ISSN":["1934-8525","1934-8533"],"issn-type":[{"value":"1934-8525","type":"print"},{"value":"1934-8533","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,2]]}}}