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Nanocarriers preserve therapeutics\u2019 bioavailability and reduce systemic toxicity, while bacteria selectively colonize the cancerous tissue, impart intrinsic and immune\u2010mediated antitumor effects, and propel nanotherapeutics interstitially. The optimal bacteria\u2013nanoparticle (NP) conjugates will carry the maximal NP load with minimal motility speed hindrance for effective interstitial distribution. Furthermore, a well\u2010defined and repeatable NP attachment density distribution is crucial to determining these biohybrid systems\u2019 efficacious dosage and robust performance. Herein, our nanoscale bacteria\u2010enabled autonomous delivery system (NanoBEADS) platform is utilized to investigate the effects of assembly process parameters of mixing method, volume, and duration on NP attachment density and repeatability. The effect of linkage chemistry and NP size on NP attachment density, viability, growth rate, and motility of NanoBEADS is also evaluated. It is shown that the linkage chemistry impacts NP attachment density while the self\u2010assembly process parameters affect the repeatability and, to a lesser extent, attachment density. Lastly, the attachment density affects NanoBEADS\u2019 growth rate and motility in an NP size\u2010dependent manner. These findings will contribute to the development of scalable and repeatable bacteria\u2013NP biohybrids for applications in drug delivery and beyond. 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