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The focus is on CAP's therapeutic potential, particularly its ability to generate reactive oxygen and nitrogen species (RONS) that play a crucial role in antimicrobial activity. RGB, HSV, LAB, YCrCb, and grayscale color spaces are extracted from the colorimetric expression of oxidative stress induced by RONS, and these features are used for unsupervised ML, employing density\u2010based spatial clustering of applications with noise (DBSCAN). The DBSCAN model's performance is evaluated using homogeneity, completeness, and adjusted rand index with a predictive data distribution graph. The best results are achieved with 3,3\u2032,5,5\u2032\u2010tetramethylbenzidine\u2013potassium iodide colorimetric assay solution immediately after plasma treatment, with values of 0.894, 0.996, and 0.826. t\u2010SNE is further conducted for the best\u2010case scenario to evaluate the clustering efficacy and find the best combination of features to better present the results. 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Through this innovative fusion, complex relationships are unraveled, marking a paradigm shift in biomedical analytical methodologies.<\/jats:p>","DOI":"10.1002\/aisy.202400029","type":"journal-article","created":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T00:49:36Z","timestamp":1719535776000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Unveiling the Potential: Can Machine Learning Cluster Colorimetric Images of Cold Atmospheric Plasma Treatment?"],"prefix":"10.1002","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3682-3733","authenticated-orcid":false,"given":"Gizem Dilara","family":"Ozdemir","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering Faculty of Engineering and Architecture Izmir Katip Celebi University  Izmir 35620 Cigli Turkey"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8758-113X","authenticated-orcid":false,"given":"Mehmet Akif","family":"Ozdemir","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Faculty of Engineering and Architecture Izmir Katip Celebi University  Izmir 35620 Cigli Turkey"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2421-9184","authenticated-orcid":false,"given":"Mustafa","family":"Sen","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Faculty of Engineering and Architecture Izmir Katip Celebi University  Izmir 35620 Cigli Turkey"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9762-2265","authenticated-orcid":false,"given":"Utku K\u00fcr\u015fat","family":"Ercan","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Faculty of Engineering and Architecture Izmir Katip Celebi University  Izmir 35620 Cigli Turkey"}]}],"member":"311","published-online":{"date-parts":[[2024,6,27]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21082932"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mattod.2022.03.001"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1063\/5.0008093"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2022.05.014"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2021.01.001"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-07598-2"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ppap.202200246"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ifset.2022.103265"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/1758835918786475"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1615\/PlasmaMed.2018026881"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snr.2019.100001"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-022-12252-y"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bspc.2022.103787"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neucom.2015.11.046"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfranklin.2015.11.013"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1814259"},{"key":"e_1_2_10_18_1","first-page":"2022","volume":"12","author":"Strzelecki M.","year":"2022","journal-title":"Mach. 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