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Biomaterial screens are typically fabricated as microarrays or \u201cchips,\u201d seeded with the cell type of interest, then phenotyped using immunocytochemistry and high\u2010content imaging, generating vast quantities of image data. Typically, analysis is only performed on fluorescent cell images as it is relatively simple to automate through intensity thresholding of cellular features. Automated analysis of bright\u2010field images is rarely performed as it presents an automation challenge as segmentation thresholds that work in all images cannot be defined. This limits the biological insight as cell response cannot be correlated to specifics of the biomaterial feature (e.g., shape, size) as these features are not visible on fluorescence images. Computer Vision aims to digitize tasks humans do by sight, such as identify objects by their shape. Herein, two case studies demonstrate how open\u2010source approaches, (region\u2010based convolutional neural network and algorithmic [OpenCV]), can be integrated into cell\u2010biomaterial HTS analysis to automate bright\u2010field segmentation across thousands of images, allowing rapid, spatial definition of biomaterial features during cell analysis for the first time.<\/jats:p>","DOI":"10.1002\/aisy.202400573","type":"journal-article","created":{"date-parts":[[2024,8,22]],"date-time":"2024-08-22T07:27:23Z","timestamp":1724311643000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Computer Vision for Substrate Detection in High\u2010Throughput Biomaterial Screens Using Bright\u2010Field Microscopy"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1961-0733","authenticated-orcid":false,"given":"Robert","family":"Owen","sequence":"first","affiliation":[{"name":"School of Pharmacy Faculty of Science University of Nottingham  Nottingham NG7 2RD UK"},{"name":"Biodiscovery Institute University of Nottingham  Nottingham NG7 2RD UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0500-9974","authenticated-orcid":false,"given":"Aishah","family":"Nasir","sequence":"additional","affiliation":[{"name":"Biodiscovery Institute University of Nottingham  Nottingham NG7 2RD UK"},{"name":"Division of Cancer &amp; Stem Cells Faculty of Medicine &amp; Health Sciences University of Nottingham  Nottingham NG7 2RD UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1493-3181","authenticated-orcid":false,"given":"Mahetab H.","family":"Amer","sequence":"additional","affiliation":[{"name":"School of Pharmacy Faculty of Science University of Nottingham  Nottingham NG7 2RD UK"},{"name":"Division of Cell Matrix Biology and Regenerative Medicine School of Biological Sciences Faculty of Biology Medicine and Health University of Manchester  Manchester M13 9NT UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenxue","family":"Nie","sequence":"additional","affiliation":[{"name":"School of Computer Science Faculty of Science University of Nottingham  Nottingham NG8 1BB UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6569-6852","authenticated-orcid":false,"given":"Xuan","family":"Xue","sequence":"additional","affiliation":[{"name":"School of Pharmacy Faculty of Science University of Nottingham  Nottingham NG7 2RD UK"},{"name":"Department of Chemistry Xi'an Jiaotong\u2010Liverpool University  Suzhou Industrial Park 215123 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0389-5148","authenticated-orcid":false,"given":"Laurence","family":"Burroughs","sequence":"additional","affiliation":[{"name":"School of Pharmacy Faculty of Science University of Nottingham  Nottingham NG7 2RD UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0802-8617","authenticated-orcid":false,"given":"Chris","family":"Denning","sequence":"additional","affiliation":[{"name":"Biodiscovery Institute University of Nottingham  Nottingham NG7 2RD UK"},{"name":"Division of Cancer &amp; Stem Cells Faculty of Medicine &amp; Health Sciences University of Nottingham  Nottingham NG7 2RD UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2329-8471","authenticated-orcid":false,"given":"Ricky D.","family":"Wildman","sequence":"additional","affiliation":[{"name":"Centre for Additive Manufacturing Faculty of Engineering University of Nottingham  Nottingham NG8 1BB UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faraz A.","family":"Khan","sequence":"additional","affiliation":[{"name":"Digital and Technology Services (DTS) University of Nottingham  Nottingham NG7 2RD UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5182-493X","authenticated-orcid":false,"given":"Morgan R.","family":"Alexander","sequence":"additional","affiliation":[{"name":"School of Pharmacy Faculty of Science University of Nottingham  Nottingham NG7 2RD UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6640-8840","authenticated-orcid":false,"given":"Felicity R. 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