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Observations on all subdivisions of the brain were compared with immersion\u2010fixed Golgi preparations and formalin\u2010perfused brains prepared by Nissl or reduced silver methods. Perfusion\u2010fixation yielded more uniform and extensive Golgi impregnations, which were more representative of the varieties of neural structures than in immersion\u2010fixed preparations. The morphological details of fine neuronal processes, delicate dendritic branchlets and spines, and fine elements of the axonal plexuses were more clearly and consistently demonstrable. Observations with the polarizing microscope suggested that the Golgi impregnations contained silver dichromate and\/or silver monochromate. The procedures and the predictability, nature, and histological reliability of the rapid Golgi method are discussed.<\/jats:p>","DOI":"10.1002\/aja.1001180309","type":"journal-article","created":{"date-parts":[[2005,2,27]],"date-time":"2005-02-27T04:02:34Z","timestamp":1109476954000},"page":"811-831","source":"Crossref","is-referenced-by-count":108,"title":["Perfusion\u2010fixation of the brain with chrome\u2010osmium solutions for the rapid Golgi method"],"prefix":"10.1002","volume":"118","author":[{"given":"D. Kent","family":"Morest","sequence":"first","affiliation":[]},{"given":"R. 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