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Selected stages were also studied in histochemical preparations for aliesterase and formaldehyde\u2010induced monoamine fluorescence, as well as by scanning and transmission electron microscopy. Neuroepithelial bodies (NEBs) first appear in pseudoglandular lungs at 15 days <jats:italic>in vivo<\/jats:italic> as pyramidal groups of basal, diffusely lead\u2010hematoxylin\u2010positive cells in glycogen\u2010depleted epithelium of main and lobar bronchi. By day 16, primitive NEBs occur within three to four generations of the terminal buds, and older, proximal bodies are larger and more distinctive than at 15 days. Aliesterase activity is first detected in basally located, developing NEBs on day 16. During the canalicular and alveolar sac periods, NEBs appear and mature on a proximal\u2010to\u2010distal gradient along the airway, as they do in developing rabbit and human lungs. As earlier\u2010formed airways elongate, additional NEBs appear and supplement the population already present. By days 20\u201321, NEBs occur at all airway levels down to the bronchiolo\u2010alveolar junctions, and many of the cells have discrete PAS\u2010 and lead\u2010hematoxylin\u2010positive, infranuclear granules. Near term some NEBs exhibit serotonin fluorescence after incubation in 5\u2010hydroxytryptophan and have abundant, ca. 100\u2010nm, electron\u2010dense granules. These are concentrated toward the cell base like the stained granules visualized by light microscopy. Similar results were obtained from lungs placed in organ culture. From 2 days in culture to a time equivalent to term, NEB formation parallels that <jats:italic>in vivo<\/jats:italic>, indicating that developmental requirements are met in <jats:italic>in vitro<\/jats:italic>. Taken altogether, morphologic and cytochemical evidence suggests that NEBs of rats are functional in late fetal life and that their development is relatively independent of extrapulmonary influences and of the intraepithelial ingrowth of sensory nerve endings.<\/jats:p>","DOI":"10.1002\/aja.1001730102","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T17:18:46Z","timestamp":1109438326000},"page":"1-27","source":"Crossref","is-referenced-by-count":40,"title":["Development of neuroepithelial bodies in intact and cultured lungs of fetal rats"],"prefix":"10.1002","volume":"173","author":[{"given":"Victor Hugo","family":"Carabba","sequence":"first","affiliation":[]},{"given":"Sergei Pitirimovitch","family":"Sorokin","sequence":"additional","affiliation":[]},{"suffix":"Jr.","given":"Richard Francis","family":"Hoyt","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,2,3]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"196","article-title":"Immunocytochemical localization of calcitonin in Kultchitsky cells of human lungs","volume":"104","author":"Becker K. 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