{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T16:00:28Z","timestamp":1775232028498,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,3]],"date-time":"2005-02-03T00:00:00Z","timestamp":1107388800000},"content-version":"vor","delay-in-days":4051,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Islet\u20101 (<jats:italic>Isl<\/jats:italic>\u2010<jats:italic>1<\/jats:italic>) is a LIM domain\/homeodomain\u2010type transcription regulator that has been originally identified as an insulin gene enhancer binding protein. <jats:italic>Isl<\/jats:italic>\u2010<jats:italic>1<\/jats:italic> is also expressed by subsets of neurons in the central nervous system of rat and chick embryos. We have cloned the <jats:italic>Isl<\/jats:italic>\u2010<jats:italic>1<\/jats:italic> cDNA from zebrafish and examined its expression pattern using in situ hybridization to whole\u2010mount embryos. <jats:italic>Isl<\/jats:italic>\u2010<jats:italic>1<\/jats:italic> mRNA first appears immediately after gastrulation in the polster, the cranial ganglia, and in Rohon\u2010Beard neurons and ventromedial cells of the spinal cord. The expression by the ventromedial cells is segmentally repeated and becomes restricted to the one or two cells slightly anterior to the segment borders. Double staining by in situ hybridization and an antibody which stains most axons suggested that these segmentally distributed cells may be either the rostral primary motoneuron (RoP) or middle primary motoneuron (MiP). This raises a possibility that <jats:italic>Isl<\/jats:italic>\u2010<jats:italic>1<\/jats:italic> may be involved during determination of subtype identities of the primary motoneurons. Furthermore, the specific <jats:italic>Isl<\/jats:italic>\u2010<jats:italic>1<\/jats:italic> mRNA expression in the spinal cord is under the control of the somites, since mutant embryo with defective somite failed to maintain this pattern. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/aja.1001990102","type":"journal-article","created":{"date-parts":[[2005,2,26]],"date-time":"2005-02-26T11:34:43Z","timestamp":1109417683000},"page":"1-11","source":"Crossref","is-referenced-by-count":210,"title":["Developmental regulation of Islet\u20101 mRNA expression during neuronal differentiation in embryonic zebrafish"],"prefix":"10.1002","volume":"199","author":[{"given":"Akihiro","family":"Inoue","sequence":"first","affiliation":[]},{"given":"Mika","family":"Takahashi","sequence":"additional","affiliation":[]},{"given":"Kohei","family":"Hatta","sequence":"additional","affiliation":[]},{"given":"Yoshiki","family":"Hotta","sequence":"additional","affiliation":[]},{"given":"Hitoshi","family":"Okamoto","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,2,3]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903020315"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90192-G"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.10-06-01892.1990"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.6.5.715"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1708527"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(91)90173-W"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/320269a0"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1350865"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/344876a0"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1242\/dev.103.1.49","article-title":"Segmental pattern of development of the hindbrain and spinal cord of the zebrafish embryo","volume":"103","author":"Hanneman E.","year":"1988","journal-title":"Development"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(89)90200-6"},{"key":"e_1_2_1_14_1","unstructured":"Hatta K. andKimmel C. B.(1993) Midline structures and CNS co\u2010ordinates in zebrafish.Perspect. Neural Dev.(in press)."},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.900670303"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/348728a0"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(93)90156-J"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"10613","DOI":"10.1016\/S0021-9258(19)50061-8","article-title":"Cloning and sequence analysis of cDNA for neuronal cell membrane antigen, HPC\u20101","volume":"267","author":"Inoue A.","year":"1992","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/344879a0"},{"key":"e_1_2_1_20_1","volume-title":"Stages of Early Development of the Zebrafish","author":"Kimmel C. 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