{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:44:30Z","timestamp":1761057870766},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2003,12,30]],"date-time":"2003-12-30T00:00:00Z","timestamp":1072742400000},"content-version":"vor","delay-in-days":5356,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Angew. Chem. Int. Ed. Engl."],"published-print":{"date-parts":[[1989,5]]},"abstract":"<jats:p><jats:bold>Lantibiotics are peptide antibiotics which contain lanthionine.<\/jats:bold> Pep 5, a strongly basic compound, is produced from <jats:italic>Staphylococcus epidermidis 5<\/jats:italic> by ribosomal synthesis of a precursor peptide and post\u2010translational modification. The antibiotic activity of Pep 5 rests on the formation of strain\u2010dependent pores in bacterial membranes. Pep 5 is the hitherto largest lantibiotic (<jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub> = 3488). It is <jats:italic>N<\/jats:italic>\u2010terminally blocked by a 2\u2010oxobutyryl residue and contains, besides 25 protein amino acids, dehydrobutyrine (2), <jats:italic>meso<\/jats:italic>\u2010lanthionine (2) and 3\u2010methyllanthionine (1). Automatic Edman degradation, mass spectrometry of enzymatically obtained fragments and chemical derivatization gave a 34\u2010peptide sequence with three sulfide rings (lanthionine = S(CH<jats:sub>2<\/jats:sub>\uf8ffCHNH<jats:sub>2<\/jats:sub>\uf8ffCOOH)<jats:sub>2<\/jats:sub>).<\/jats:p>","DOI":"10.1002\/anie.198906161","type":"journal-article","created":{"date-parts":[[2003,12,31]],"date-time":"2003-12-31T08:46:57Z","timestamp":1072860417000},"page":"616-619","source":"Crossref","is-referenced-by-count":65,"title":["Pep5: Structure Elucidation of a Large Lantibiotic"],"prefix":"10.1002","volume":"28","author":[{"given":"Roland","family":"Kellner","sequence":"first","affiliation":[]},{"given":"G\u00fcnther","family":"Jung","sequence":"additional","affiliation":[]},{"given":"Michaela","family":"Josten","sequence":"additional","affiliation":[]},{"given":"Cortina","family":"Kaletta","sequence":"additional","affiliation":[]},{"given":"Karl\u2010Dieter","family":"Entian","sequence":"additional","affiliation":[]},{"given":"Hans\u2010Georg","family":"Sahl","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2003,12,30]]},"reference":[{"key":"e_1_2_1_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/333276a0"},{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja00747a073"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2164(08)70342-3"},{"key":"e_1_2_1_4_2","first-page":"810","volume":"354","author":"Gross E.","year":"1973","journal-title":"Hoppe\u2010Seyler's Z. Physiol. Chem."},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-9113-6_9"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4039(00)89174-1"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.19850971214"},{"key":"e_1_2_1_7_3","doi-asserted-by":"publisher","DOI":"10.1002\/anie.198510511"},{"key":"e_1_2_1_7_4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1986.tb09933.x"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/hlca.19870700322"},{"key":"e_1_2_1_8_3","doi-asserted-by":"publisher","DOI":"10.1002\/hlca.19880710811"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1988.tb14344.x-i2"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2787(70)90642-8"},{"key":"e_1_2_1_10_3","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(83)90588-0"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)76571-5"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)37587-2"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.3.1597-1601.1989"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1989.tb03056.x"},{"key":"e_1_2_1_15_2","first-page":"377","volume":"127","author":"Sahl H.\u2010G.","year":"1981","journal-title":"J. Gen. Microbiol."},{"key":"e_1_2_1_15_3","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.27.5.836"},{"key":"e_1_2_1_15_4","doi-asserted-by":"publisher","DOI":"10.1128\/jb.170.1.84-88.1988"},{"key":"e_1_2_1_16_2","unstructured":"C.Kaletta K.\u2010D.Entian R.Kellner G.Jung M.Reis H.\u2010G.Sahl Arch. Microbiol. in press."},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02259079"},{"key":"e_1_2_1_18_2","unstructured":"For automated Edman degradation a pulsed liquid\u2010phase sequencer Model 477 A with an on\u2010line Model 120 PTH\u2010analyzer (Applied Biosystems) was used. Sequencing reagents and solvents were from Applied Biosystems. The acidic total hydrolysates were analyzed by gas chromatographic enantiomer analysis on the chiral phase Chirasil\u2010Val [17] using a Sichromat Autoderivat 100 (Siemens). FAB\u2010Mass spectra were recorded with a VG 70\/250\/SEQ and the samples were applied in a matrix of 3\u2010nitrobenzylalcohol\/methanol."},{"key":"e_1_2_1_19_2","first-page":"807","volume":"354","author":"Nebelin E.","year":"1973","journal-title":"Hoppe\u2010Seyler's Z. Physiol. Chem."},{"key":"e_1_2_1_19_3","doi-asserted-by":"publisher","DOI":"10.1021\/ja00712a055"}],"container-title":["Angewandte Chemie International Edition in English"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fanie.198906161","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/anie.198906161","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T13:16:39Z","timestamp":1697980599000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/anie.198906161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,5]]},"references-count":26,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1989,5]]}},"alternative-id":["10.1002\/anie.198906161"],"URL":"https:\/\/doi.org\/10.1002\/anie.198906161","archive":["Portico"],"relation":{},"ISSN":["0570-0833"],"issn-type":[{"value":"0570-0833","type":"print"}],"subject":[],"published":{"date-parts":[[1989,5]]}}}