{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T17:11:43Z","timestamp":1771002703046,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,11,22]],"date-time":"2005-11-22T00:00:00Z","timestamp":1132617600000},"content-version":"vor","delay-in-days":11102,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[1975,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A nonimmunoglobulin protein (A protein) has been isolated from amyloidotic tissue of secondary type. Antisera prepared to this protein identified a cross\u2010reacting substance in the sera of patients with secondary amyloidosis. Sera from 70 persons with amyloidosis, 120 normal adults, 20 aged persons, and 97 patients with chronic diseases were tested for this substance. One hundred percent of secondary amyloid sera had amounts of amyloid serum component detectable by double diffusion in agar, whereas only 19% of primary amyloid sera were positive. Approximately 60% of rheumatoid sera as well as 60% of sera from aged individuals were positive. Only 3% of normal blood donors had detectable amounts of this circulating substance. Isolation of the serum component by affinity chromatography and partial characterization have shown that it is an \u03b1\u2010globulin with a molecular weight of 100,000\u2013120,000, that it is not related antigenically to immunoglobulin or amyloid P\u2010component, and that it has an amino acid analysis that is markedly different from tissue A protein. The possible participation of this substance in the genesis of amyloid is discussed.<\/jats:p>","DOI":"10.1002\/art.1780180404","type":"journal-article","created":{"date-parts":[[2007,2,14]],"date-time":"2007-02-14T16:27:57Z","timestamp":1171470477000},"page":"315-322","source":"Crossref","is-referenced-by-count":27,"title":["\u201cA\u201d protein of amyloidosis isolation of a cross\u2010reacting component from serum by affinity chromatography"],"prefix":"10.1002","volume":"18","author":[{"given":"Merrill D.","family":"Benson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martha","family":"Skinner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane","family":"Lian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan S.","family":"Cohen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00766a002"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107098"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"5653","DOI":"10.1016\/S0021-9258(20)81154-5","article-title":"Amino acid sequence of an amyloid fibril protein of unknown origin.","volume":"247","author":"Ein D","year":"1972","journal-title":"J Biol Chem"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1972.tb03305.x"},{"key":"e_1_2_1_6_2","unstructured":"BensonMD SkinnerM CohenAS:Amyloid fibril proteins: antigenicity and cross\u2010reactivity of denatured fibril proteins of primary secondary and myeloma associated amyloids (submitted for 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reliability of molecular weight determinations by dodecyl sulfate\u2010polyacrylamide gel electrophoresis.","volume":"244","author":"Weber K","year":"1969","journal-title":"J Biol Chem"},{"key":"e_1_2_1_20_2","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1021\/ac60139a006","article-title":"Automatic recording apparatus for use in the chromatography of amino acids.","volume":"30","author":"Spackman DH","year":"1958","journal-title":"Anal Chem"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2795(67)90420-5"},{"key":"e_1_2_1_22_2","first-page":"159","article-title":"The constitution and genesis of amyloid.","volume":"4","author":"Cohen AS","year":"1966","journal-title":"Int Rev Exp Pathol"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107642"},{"key":"e_1_2_1_24_2","first-page":"438","volume-title":"The incidence of amyloid deposits in 100 consecutive post mortem examinations, Proceedings of the Symposium on 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