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Over the past two decades, it has become increasingly clear that aspects of this three\u2010dimensionality which reflect topological relationships within the double helix (i.e., superhelical twisting, knotting, or tangling) influence virtually every facet of nucleic acid physiology. <jats:italic>In vivo<\/jats:italic>, DNA topology is modulated by ubiquitous enzymes known as topoisomerases. The type II enzyme essential to the eukaryotic cell and is required for unlinking daughter chromosomes and maintaining chromosome structure. Moreover, topoisomerase II also has been identified as the primary cellular target for several widely used antineoplastic drugs. Before the physiological functions of topoisomerase II can be effectively dissected or its drug interactions fully exploited, it is imperative to understand the mechanism by which this important enzyme arries out its catalytic cycle.<\/jats:p>","DOI":"10.1002\/bies.950130603","type":"journal-article","created":{"date-parts":[[2005,2,25]],"date-time":"2005-02-25T11:16:02Z","timestamp":1109330162000},"page":"269-275","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":185,"title":["Catalytic function of DNA topoisomerase II"],"prefix":"10.1002","volume":"13","author":[{"given":"Neil","family":"Osheroff","sequence":"first","affiliation":[]},{"given":"E. 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