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Although powerful techniques, they leave many soluble, purified structurally uncharacterized protein samples. Small angle X\u2010ray scattering (SAXS) is a solution technique that provides data on the size and multiple conformations of a sample, and can be used to reconstruct a low\u2010resolution molecular envelope of a macromolecule. In this study, SAXS has been used in a high\u2010throughput manner on a subset of 28 proteins, where structural information is available from crystallographic and\/or NMR techniques. These crystallographic and NMR structures were used to validate the accuracy of molecular envelopes reconstructed from SAXS data on a statistical level, to compare and highlight complementary structural information that SAXS provides, and to leverage biological information derived by crystallographers and spectroscopists from their structures. All the ab initio molecular envelopes calculated from the SAXS data agree well with the available structural information. SAXS is a powerful albeit low\u2010resolution technique that can provide additional structural information in a high\u2010throughput and complementary manner to improve the functional interpretation of high\u2010resolution structures. \u00a9 2011 Wiley Periodicals, Inc. 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