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In addition, deposition of hydroxyapatite (HA) on the surface of the porous scaffolds was performed by precipitation method to achieve modified chemical compositions for chondrocyte attachments and growths. The experimental results revealed that a lower prefreezing temperature or a higher weight percentage of chitin in the chitin\u2010chitosan scaffolds would yield a smaller pore diameter, a greater porosity, a larger specific surface area, a higher Young's modulus, and a lower extensibility. Moreover, a higher chitin percentage could also result in a higher content of amine groups after crosslink and a lower onset temperature for the phase transition after thermal treatment. A decrease in the prefreezing temperature from \u22124\u00b0C to \u221280\u00b0C, an increase in the chitin percentage from 20% to 50%, and an increase in the cycle number of alternate immersion for HA deposition from 1 to 5 generated positive effects on the cell number, the content of glycosaminoglycans, and the collagen level over 28\u2010day cultivation of bovine knee chondrocytes. \u00a9 2006 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.21007","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T20:59:28Z","timestamp":1149109168000},"page":"132-144","source":"Crossref","is-referenced-by-count":68,"title":["Effect of genipin\u2010crosslinked chitin\u2010chitosan scaffolds with hydroxyapatite modifications on the cultivation of bovine knee 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