{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T01:55:35Z","timestamp":1773626135138,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2004,2,18]],"date-time":"2004-02-18T00:00:00Z","timestamp":1077062400000},"content-version":"vor","delay-in-days":5432,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[1989,4,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Extension of a single cell model of <jats:italic>E. coli<\/jats:italic> B\/r to make predictions of culture response to variations in glutamine\/glucose\/ammonium ion concentrations is described. A biphasic glutamine transport system, a nitrogen metabolism scheme that includes glutamate dehydrogenase (GDH), glutamine synthetase\/glutamate synthase (GS\/GOGAT), the glutaminase routes, and a transaminase mechanism for glutamine carbon usage are added to the prototype model. The predictions of the extended model with regard to nutrient concentrations and cell size compare well with the experimental data and the prototype model predictions, demonstrating the capability of the integrated kinetic model to illustrate important enzymological interactions in a biological system. The discrepancies between the experimental data and the model predictions on growth yield suggest that a more detailed regulatory system of the TCA cycle is required for a more accurate energy budget.<\/jats:p>","DOI":"10.1002\/bit.260330907","type":"journal-article","created":{"date-parts":[[2005,5,27]],"date-time":"2005-05-27T13:19:39Z","timestamp":1117199979000},"page":"1117-1126","source":"Crossref","is-referenced-by-count":27,"title":["A mathematical model for the growth of a single cell of <i>E. coli<\/i> on a glucose\/glutamine\/ammonium medium"],"prefix":"10.1002","volume":"33","author":[{"given":"J.","family":"Shu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. L.","family":"Shuler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,18]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"Amino Acid Metabolism","author":"Bender D. A.","year":"1975"},{"key":"e_1_2_1_3_2","first-page":"7747","volume":"249","author":"Berger E. A.","year":"1974","journal-title":"J. Bio. Dhem."},{"key":"e_1_2_1_4_2","volume-title":"Bacteriol Transport","author":"Rosen B. P.","year":"1978"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"1032","DOI":"10.1128\/jb.122.3.1032-1037.1975","volume":"122","author":"Willis R. C.","year":"1975","journal-title":"J. Bacteriol"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-69-3-299"},{"key":"e_1_2_1_7_2","first-page":"9","volume-title":"Enzymes of Glutamine Metabolism","author":"Ginsburg A.","year":"1973"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"7407","DOI":"10.1016\/S0021-9258(19)44642-5","volume":"274","author":"Miller R. E.","year":"1972","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.123.2.407-418.1975"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-152804-1.50009-X"},{"key":"e_1_2_1_11_2","unstructured":"C. M.Brown Ammonia Assimilation and Utilization in Bacteria and Fungi Microorganisms and Nitrogen Sources (1980)."},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","unstructured":"M. L.ShulerandM. M.Domach Mathematical models of the growth of individual cells inFoundations of Biochemical Engineering E. T. Papoutsakis and G. Stephanopoulos Eds. ACS Symposium Series No. 207 (1983). pp.93\u2013133.","DOI":"10.1021\/bk-1983-0207.ch005"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260260303"},{"key":"e_1_2_1_14_2","unstructured":"J. H.Weiner A Binding Protein for Glutamine and its Relation to Active Transport in Escherichia Coli Ph. D. Thesis Cornell University Ithaca NY (1972)."},{"key":"e_1_2_1_15_2","volume-title":"The Enzymes of Glutamine Metabolism","author":"Prusiner S.","year":"1973"},{"key":"e_1_2_1_16_2","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S0021-9258(18)93597-0","volume":"243","author":"Hartman S. C.","year":"1968","journal-title":"J. Bio. Chem."},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-65-1-57"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00520a015"},{"key":"e_1_2_1_19_2","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1128\/jb.132.1.100-105.1977","volume":"132","author":"Bender R. A.","year":"1977","journal-title":"J. Bacteriol"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260280209"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260300310"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2795(74)90385-7"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1978.tb12388.x"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(66)90149-8"},{"key":"e_1_2_1_25_2","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1128\/jb.169.2.553-557.1987","volume":"169","author":"Jayakumar A.","year":"1987","journal-title":"J. Bacteriol."},{"key":"e_1_2_1_26_2","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1128\/jb.166.1.281-284.1986","volume":"116","author":"Jayakumar A.","year":"1986","journal-title":"J. Bacteriol."}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbit.260330907","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.260330907","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T21:31:38Z","timestamp":1760045498000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.260330907"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,4,5]]},"references-count":25,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1989,4,5]]}},"alternative-id":["10.1002\/bit.260330907"],"URL":"https:\/\/doi.org\/10.1002\/bit.260330907","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"value":"0006-3592","type":"print"},{"value":"1097-0290","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,4,5]]}}}