{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T02:55:06Z","timestamp":1761101706630,"version":"build-2065373602"},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":3181,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[1995,6,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effect of magnesium and phosphate limitation on the molecular weight distribution of poly\u2010\u03b2\u2010hydroxybutyrate (PHB) in <jats:italic>Alcaligens europhus<\/jats:italic> in cotinuons culture has been stuied. Conditions of nitrogen limitation both with glucose excess (above ca. 20 g\/L) and without excess were investigated Under N\u2010limitation and glucose excess, <jats:italic>M<\/jats:italic><jats:sub>w<\/jats:sub> decreases when the magnesium content is decreased below 50% (19.7 mg\/L) of the basal medium content; this also results in a broadenng of molecular weight distribution (<jats:italic>M<\/jats:italic><jats:sub>w<\/jats:sub>\/<jats:italic>M<\/jats:italic><jats:sub>n<\/jats:sub>) from 2 to 5 and a decrease in <jats:italic>M<\/jats:italic><jats:sub>w<\/jats:sub> fron 2 \u00d7 10<jats:sup>6<\/jats:sup> to 0.9 \u00d7 10<jats:sup>6<\/jats:sup>. Below 20% of the basal content of magnesium (7.9 mg\/L) these two trends were reversed. This behaviour was not observed in the absence of glucose excess, phshate had virtually no effect on PHB <jats:italic>M<\/jats:italic><jats:sub>w<\/jats:sub> or its distribution, whereas wih no (or little) glucose excess <jats:italic>M<\/jats:italic><jats:sub>w<\/jats:sub> of the PHB decreased with phosphate concentrations below 50% of the basal level (0.705 g\/L). Hence, in continuous or fed\u2010batch cultures, in addition to nitrogen limitation to alklow for PHB accumulation, it is necesary to control both the addition of glucose (no excess) and also to maintain magnesium limitation (ca. 25% of basal medium level, 9.9 mg\/L) and phosphate above 50% of he basal level (0.705 g\/L). Thus, when broadening of molecular weight destribution (increase in <jats:italic>M<\/jats:italic><jats:sub>w<\/jats:sub>\/<jats:italic>M<\/jats:italic><jats:sub>n<\/jats:sub>) is observed at the end of fed\u2010batch culture it is probably caused by phosphate limitation and\/or glucose excess. \u00a9 1995 John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/bit.260460514","type":"journal-article","created":{"date-parts":[[2004,12,29]],"date-time":"2004-12-29T19:09:33Z","timestamp":1104347373000},"page":"497-502","source":"Crossref","is-referenced-by-count":22,"title":["Effect of single nutrient limitation of poly\u2010\u03b2\u2010hydroxybutyrate molecular weight distribution in alcaligens europhus"],"prefix":"10.1002","volume":"46","author":[{"given":"J. A.","family":"Asenjo","sequence":"first","affiliation":[]},{"given":"A. S.","family":"Schmidt","sequence":"additional","affiliation":[]},{"given":"P. R.","family":"Andersen","sequence":"additional","affiliation":[]},{"given":"B. A.","family":"Andrews","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_2","unstructured":"Andersen P. R.1989. Control of PHB molecular weight destribution MSc. thesis The Technical University of Denmark Lyngby and university of Reading U. K."},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0385-6380(86)90118-4"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1987.tb23807.x"},{"key":"e_1_2_1_5_2","first-page":"77","volume-title":"Advances in biochemical engineering biotechnology","author":"Brandl H.","year":"1990"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-7799(87)90100-4"},{"key":"e_1_2_1_7_2","first-page":"173","article-title":"FEMS Microbiol","volume":"84","author":"Chem G.\u2010Q.","year":"1991","journal-title":"Lett."},{"volume-title":"Microbial energetics","year":"1986","author":"Dawes E. A.","key":"e_1_2_1_8_2"},{"volume-title":"Standard methods for the examination of water and wasewater","year":"1976","author":"Franson M. A.","key":"e_1_2_1_9_2"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-6968.1989.tb03210.x","article-title":"The importance of PHB\u2010synthases substrae specificity in poyalkanoate synthesis by Alcaligenes eutrophus","volume":"57","author":"Haywood G. W.","year":"1989","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1088\/0305-4624\/16\/1\/305"},{"key":"e_1_2_1_12_2","first-page":"1","article-title":"Some aspects of environmental regulation of microbial phosphorus metabolism","volume":"23","author":"Kulaev I. S.","year":"1985","journal-title":"FEMS Symp."},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.82.1.33-36.1961"},{"key":"e_1_2_1_14_2","unstructured":"Schmidt A. S.1990. Efect of single limitation on poly\u2010\u03b2\u2010hydroxybutyrate molecular weight distribution in bacteria MSc. thesis The Technical university of Denmark and university of Reading U.K."},{"key":"e_1_2_1_15_2","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1042\/bj1340225","article-title":"The regulation of poly\u2010\u03b2\u2010hydroxybutyrate metabolism in Azotobacter beijerinckii","volume":"134","author":"Seniro P. J.","year":"1973","journal-title":"Biochem. J."},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1904627"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00257027"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00294592"},{"key":"e_1_2_1_19_2","unstructured":"Winto J. 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