{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T07:28:19Z","timestamp":1776756499354,"version":"3.51.2"},"reference-count":16,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":8800,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motility"],"published-print":{"date-parts":[[1981,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dissociated bovine brain microtubule protein has been shown to reassemble at 0\u00b0C in the presence of the drug taxol. Tubulin polymerization was monitored both by electron microscopy of the polymeric structures and by incorporation of tritiated GTP into filterable polymeric structures. Most of the labeled guanine nucleotide uptake into tubulin polymeric structures occurred in the first 30 minutes of incubation with the drug. The initial polymerization event results in the formation of protofilamentous tubulin ribbons. The first microtubules were noted after 1 hour of incubation with the drug. After 20 hours of incubation at 0\u00b0C with taxol, the bulk of the polymerized tubulin appeared to be in the form of microtubules. Cold\u2010stable tubulin rings with a mean diameter of 34 nm were present in the reaction mixture before the addition of taxol and throughout the 20\u2010hour incubation. Most of the rings were apparantly not involved in the taxol\u2010induced microtubule assembly. The results are consistant with a model whereby taxol induces an initial formation of protofilamentous ribbon structures, mostly from free tubulin dimers, and a slower subsequent folding of the ribbon structures into microtubules.<\/jats:p>","DOI":"10.1002\/cm.970010405","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T04:57:11Z","timestamp":1109221031000},"page":"445-454","source":"Crossref","is-referenced-by-count":48,"title":["Taxol induces microtubule assembly at low temperature"],"prefix":"10.1002","volume":"1","author":[{"given":"William C.","family":"Thompson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leslie","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel L.","family":"Purich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.14.3.523"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(79)90706-1"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.177.4055.1196"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.71.3.749"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-96436-7"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jss.400020228"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.60.1.153"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(80)90421-2"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jss.400020229"},{"key":"e_1_2_1_11_1","volume-title":"Methods and Perspectives in Cell Biology","author":"Kristofferson D.","year":"1981"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-80869-2"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/277665a0"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.3.1561"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1242\/jcs.15.3.495","article-title":"Configuration of flagellar microtubule subunits","volume":"15","author":"Warner F. 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