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Organelle transport was visualized in isolated lobster giant motor axons using Nomarski optics and video microscopy. The axons were permeabilized with saponin, and movement was reactivated with ATP or other nucleotides. Some slight movement was seen with ATP concentrations as low as 10 \u03bcM. The velocity and frequency of the reactivated transport increased with increasing ATP concentrations up to about 5 mM. Movement was also reactivated by deoxyadenosine triphosphate, but not by AMP\u2013PNP (a nonhydrolyzable ATP analogue), ADP, or AMP. Although other nucleotides (CTP, GTP, UTP, ITP) could reactivate transport, movement equivalent to that produced by 0.1 mM ATP was only seen with tenfold or greater concentrations of the other nucleotides. This pattern of specificity is consistent with the hypothesis that a dynein\u2010like ATPase, rather than a myosin, is involved in fast axonal transport.<\/jats:p>","DOI":"10.1002\/cm.970040205","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T04:47:06Z","timestamp":1109220426000},"page":"121-128","source":"Crossref","is-referenced-by-count":18,"title":["Nucleotide specificity for reactivation of organelle movements in permeabilized axons"],"prefix":"10.1002","volume":"4","author":[{"given":"David S.","family":"Forman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kurt J.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark W.","family":"Promersberger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark R.","family":"Adelman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/297327a0"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00840a047"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970010303"},{"key":"e_1_2_1_5_1","first-page":"379","article-title":"Fast axonal transport in lobster axons","volume":"163","author":"Allen R. 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