{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T11:50:32Z","timestamp":1700394632228},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":6974,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[1986,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We investigated the involvement of kinetochore microtubules (kMTs) in mediating chromosome\u2010to\u2010pole connections in crane\u2010fly (<jats:italic>Nephrotoma suturalis<\/jats:italic> and <jats:italic>Nephrotoma ferruginea<\/jats:italic>) spermatocytes. Two experimental treatments were used to yield spindles with reduced numbers of nonkinetochore microtubules (nkMTs). Short\u2010term (10\u201315 min) exposure of spermatocytes to 2\u00b0C caused depolymerization of the majority of nkMTs, resulting in a kMT:(kMT + nkMT) ratio of 0.76. Long\u2010term (24h) exposure to 2\u00b0C followed by recovery at 6\u00b0C resulted in a kMT:(kMT + nkMT) ratio of 0.55, the spindle having more nkMTs than a 2\u00b0C\u2010treated spindle but fewer than an untreated spindle, in which the kMT:(kMT + nkMT) ratio was 0.27. The numbers and lengths of kMTs in 6\u00b0C\u2010grown spindles were similar to those in untreated cells, suggesting that the overall inhibition of MT assembly at 6\u00b0C apparently did not affect the mechanism by which kMTs are formed. We observed most kMTs of early anaphase spindles to be long (&gt;3 \u03bcm), and many extended to the polar regions of the spindle. Thus, the crane\u2010fly spindle appears not to be as atypical as it was previously suggested to be.<\/jats:p>","DOI":"10.1002\/cm.970060408","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T04:31:13Z","timestamp":1109219473000},"page":"428-438","source":"Crossref","is-referenced-by-count":16,"title":["Kinetochore microtubules in crane\u2010fly spermatocytes: Untreated, 2\u00b0C\u2010treated and 6\u00b0C\u2010grown spindles"],"prefix":"10.1002","volume":"6","author":[{"given":"Lisa A.","family":"Scarcello","sequence":"first","affiliation":[]},{"given":"Marie A.","family":"Janicke","sequence":"additional","affiliation":[]},{"suffix":"Jr.","given":"James R.","family":"LaFountain","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.82.2.542"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1975.tb19218.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.93.2.365"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1242\/jcs.79.1.39","article-title":"The kinetic polarities of spindle microtubules in vivo, in crane fly spermatocytes. 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