{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T15:35:59Z","timestamp":1772897759892,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":6974,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[1986,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>When outer\u2010row dynein arms are extracted from <jats:italic>Chlamydomonas<\/jats:italic> flagellar axonemes, they dissociate into two ATPase complexes with sedimentation coefficients of 12S and 18S. We immunized mice with 18S dynein and generated a library of monoclonal antibodies against the polypeptides in this complex. Antibodies were selected which specifically recognize the 18S \u03b1\u2010 and \u03b2\u2010heavy chains and the 83,000\u2010dalton and 70,000\u2010dalton intermediate chains. These antibodies were isolated and characterized for their ability to recognize determinants on both denatured antigens and native 18S dynein; 18S dynein was dissociated in stepwise fashion into smaller aggregates with ionic and nonionic detergents and the resulting subcomplexes were isolated by precipitation with specific monoclonal antibodies. The smallest aggregates isolated were heterodimers between the \u03b1\u2010chain and a 16,000\u2010dalton light chain and between the two intermediate chains. Additional close associations of the \u03b2\u2010heavy chain with an 18,000\u2010dalton light chain and 70,000\u2010dalton intermediate chain, and a weaker interaction between the intermediate chain heterodimer and light chains of 21,000 daltons and 12,500 daltons, were also observed. We present a model of 18S dynein substructure based upon this information.<\/jats:p>","DOI":"10.1002\/cm.970060510","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T04:29:20Z","timestamp":1109219360000},"page":"510-520","source":"Crossref","is-referenced-by-count":61,"title":["Protein\u2010protein interactions in the 18S ATPase of <i>Chlamydomonas<\/i> outer dynein arms"],"prefix":"10.1002","volume":"6","author":[{"given":"David R.","family":"Mitchell","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joel L.","family":"Rosenbaum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jss.400110305"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(81)90281-5"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(84)90272-9"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(82)90677-7"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2795(74)90073-7"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"3091","DOI":"10.1016\/S0021-9258(17)30186-2","article-title":"Paralyzed flagella mutants of Chlamydomonas reinhardtii defective for axonemal microtubute arms","volume":"254","author":"Huang B.","year":"1979","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_8_1","first-page":"314","volume-title":"Practical Immunology","author":"Hudson L.","year":"1980"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"13825","DOI":"10.1016\/S0021-9258(17)43992-5","article-title":"The pathway of ATP hydrolysis by dynein","volume":"258","author":"Johnson K. A.","year":"1983","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_10_1","first-page":"181","article-title":"Structural and mass analysis of dynein by scanning transmission electron microscopy","volume":"15","author":"Johnson K. A.","year":"1983","journal-title":"J. Submicrosc. Cytol."},{"key":"e_1_2_1_11_1","first-page":"77","article-title":"Hybrid plasmacytoma production: Fusions with adult spleen cells, monoclonal spleen fragments, neonatal spleen cells, and human spleen cells","volume":"81","author":"Kennett R. H.","year":"1978","journal-title":"Curr. Top. Microb. 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K.","year":"1985","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"12072","DOI":"10.1016\/S0021-9258(20)71321-9","article-title":"Subfractionation of Chlamydomonas 18S dynein into two unique subunits containing ATPase activity","volume":"259","author":"Pfister K. K.","year":"1984","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"3084","DOI":"10.1016\/S0021-9258(17)30185-0","article-title":"Axonemal adenosine triphosphatas from flagella of Chlamydomonas reinhardtii","volume":"254","author":"Piperno G.","year":"1979","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.41.2.600"},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"13841","DOI":"10.1016\/S0021-9258(17)43994-9","article-title":"Kinetic evidence for multiple dynein ATPase cites","volume":"258","author":"Shimizu T.","year":"1983","journal-title":"J. Biol. 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