{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T10:54:27Z","timestamp":1761216867301},"reference-count":44,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":6244,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[1988,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mitotic apparatuses (MAs) isolated from sea urchin metaphase eggs were chilled on ice to depolymerize microtubules, homogenized, and incubated with tubulin. This caused formation of many small asters with microtubules focusing on granules which were probably fragments of the centrosome. The aster\u2010forming protein components of the granules in the homogenized MAs were solubilized in 0.5 M KCl containing 50% glycerol. After dialysis against low\u2010ionic\u2010strength buffer solution, proteins congregated to form granular assembly capable of initiating aster formation. Phosphocellulose column chromatography enabled the separation of the aster\u2010forming protein fraction which contained a 51,000 molecular weight protein (51\u2010kd protein) as a major component. The protein fraction possessing the aster\u2010forming activity was also prepared from methaphase whole egg homogenate, and the elution profile of the 51\u2010kd protein on phosphocellulose column also coincided with that of the aster\u2010forming activity. The granular assembly reconstituted from the phosphocellulose fraction formed asters whose microtubules show the same growth rate and length distribution as those of asters reconstructed from the granules in the homogenized MAs. Anti\u201051\u2010kd protein antibody that was raised in rabbit and affinity\u2010purified stained the center of asters which were reconstructed either from the granules in the homogenized MAs or from the granular assembly reconstituted from the phosphocellulose fraction. These results suggest that the 51\u2010kd protein is a component in the aster\u2010forming activity of the centrosomal component in vitro.<\/jats:p>","DOI":"10.1002\/cm.970090204","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T04:00:46Z","timestamp":1109217646000},"page":"117-128","source":"Crossref","is-referenced-by-count":46,"title":["51\u2010kd protein, a component of microtubule\u2010organizing granules in the mitotic apparatus involved in aster formation in vitro"],"prefix":"10.1002","volume":"9","author":[{"given":"Masaru","family":"Toriyama","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kunihiro","family":"Ohta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sachiko","family":"Endo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hikoichi","family":"Sakai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.84.1.151"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1146\/annurev.cb.01.110185.001045","article-title":"Microtubule organizing centers","volume":"1","author":"Brinkley B. 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