{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T07:05:18Z","timestamp":1762067118546},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":6244,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[1988,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ca<jats:sup>2+<\/jats:sup> \u2010ATPase of the sarcoplasmic reticulum was localized in cryostat sections from three different adult canine skeletal muscles (gracilis, extensor carpi radialis, and superficial digitalis flexor) by immunofluorescence labeling with monoclonal antibodies to the Ca<jats:sup>2+<\/jats:sup> \u2010ATPase Type I (slow) myofibers were strongly labeled for the Ca<jats:sup>2+<\/jats:sup> \u2010ATPase with a monoclonal antibody (II D8) to the CA<jats:sup>2+<\/jats:sup> \u2010ATPase of canine cardiac sarcoplasmic reticulum; the type II (fast) myofibers were labeled at the level of the background with monoclonal antibody II D8. By contrast, type II (fast) myofibers were strongly labeled for Ca<jats:sup>2+<\/jats:sup> \u2010ATPase of rabbit skeletal sarcoplasmic reticulum. The subcellular distribution of the immunolabeling in type I (slow) myofibers with monoclonal antibody II D8 corresponded to that of the sarcoplasmic reticulum as previously determined by electron microscopy. The structural similarity between the canine cardiac Ca<jats:sup>2+<\/jats:sup> \u2010ATPase present in the sarcoplasmic reticulum of the canine slow skeletal muscle fibers was demonstrated by immunoblotting. Monoclonal antibody (II D8) to the cardiac Ca<jats:sup>2+<\/jats:sup> \u2010ATPase binds to only one protein band present in the extract from either cardiac or type I (slow) skeletal muscle tissue. By contrast, monoclonal antibody (II H11) to the skeletal type II (fast) Ca<jats:sup>2+<\/jats:sup> \u2010ATPase binds only one protein band in the extract from type II (fast) skeletal muscle tissue. These immunopositive proteins coelectrophoresed with the Ca<jats:sup>2+<\/jats:sup> \u2010ATPase of the canine cardiac sarcoplasmic reticulum and showed an apparent M<jats:sub>r<\/jats:sub> of 115,000. It is concluded that the Ca<jats:sup>2+<\/jats:sup> \u2010ATPase of cardiac and type I (slow) skeletal sarcoplasmic reticulum have at least one epitope in common, which is not present on the Ca<jats:sup>2+<\/jats:sup> \u2010ATPase of sarcoplasmic reticulum in type II (fast) skeletal myofibers. It is possible that this site is related to the assumed necessity of the Ca<jats:sup>2+<\/jats:sup> \u2010ATPase of the sarcoplasmic reticulum in cardiac and type I (slow) skeletal myofibers to interact with phosphorylated phospholamban and thereby enhance the accumulation of Ca<jats:sup>2+<\/jats:sup> in the lumen of the sarcoplasmic reticulum following \u03b2\u2013adrenergic stimulation.<\/jats:p>","DOI":"10.1002\/cm.970090208","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T04:00:46Z","timestamp":1109217646000},"page":"164-174","source":"Crossref","is-referenced-by-count":74,"title":["A monoclonal antibody to the Ca<sup>2+<\/sup> \u2010ATPase of cardiac sarcoplasmic reticulum cross\u2010reacts with slow type I but not with fast type II canine skeletal muscle fibers: An immunocytochemical and immunochemical study"],"prefix":"10.1002","volume":"9","author":[{"given":"Annelise O.","family":"Jorgensen","sequence":"first","affiliation":[]},{"given":"Wayne","family":"Arnold","sequence":"additional","affiliation":[]},{"given":"David R.","family":"Pepper","sequence":"additional","affiliation":[]},{"given":"Steven D.","family":"Kahl","sequence":"additional","affiliation":[]},{"given":"Frederick","family":"Mandel","sequence":"additional","affiliation":[]},{"given":"Kevin P.","family":"Campbell","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001630107"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90269-2"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)69481-0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1970245"},{"key":"e_1_2_1_6_1","first-page":"73","volume-title":"Handbook of Physiology, Section 10, Skeletal Muscle","author":"Eisenberg B. 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