{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T07:05:16Z","timestamp":1762067116774},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":5878,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[1989,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We recently purified a calcium\u2010dependent but calmodulin\u2010 and phospholipid\u2010independent protein kinase (CDPK) from cultured plant cells (Harmon et al.: <jats:italic>Plant Physiology<\/jats:italic> 83:830\u2013837, 1987). A monoclonal antibody (mAb 3B9) directed against CDPK was used to localize this protein in <jats:italic>Allium<\/jats:italic> root cells and <jats:italic>Tradescantia<\/jats:italic> pollen tubes using immunofluorescence techniques. The mAb 3B9 staining pattern showed that CDPK is localized within a fibrous network in the cytoplasm resembling the normal interphase network of F\u2010actin. Treatment of tissue with 10 \u03bcM cytochalasin D (CD) prior to fixation abolished the staining pattern. Double\u2010localization experiments in which pollen tubes were first stained with mAb 3B9 and then with rhodamine\u2010phalloidin (RP) demonstrated that CDPK and F\u2010actin were colocalized. Monoclonal antibody 3B9 did not react with purified actin from rabbit muscle or <jats:italic>Dictyostelium<\/jats:italic> and did not bind to proteins corresponding to the M<jats:sub>r<\/jats:sub> of actin in crude extracts of <jats:italic>Allium<\/jats:italic> root tips and <jats:italic>Tradescantia<\/jats:italic> pollen tubes.<\/jats:p><jats:p>CDPK did not phosphorylate purified rabbit muscle or <jats:italic>Dictyostelium<\/jats:italic> actin in vitro. Binding studies showed that CDPK (1) does not cosediment with actin filaments and (2) does not form a complex with G\u2010actin. The data indicate that although CDPK does not interact directly with actin, it may be associated with an actin\u2010binding protein and therefore could play a role in the regulation of the plant cytoskeleton.<\/jats:p>","DOI":"10.1002\/cm.970120103","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T03:57:11Z","timestamp":1109217431000},"page":"12-22","source":"Crossref","is-referenced-by-count":87,"title":["Calcium\u2010dependent protein kinase is localized with F\u2010actin in plant cells"],"prefix":"10.1002","volume":"12","author":[{"given":"Cindy","family":"Putnam\u2010Evans","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alice C.","family":"Harmon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barry A.","family":"Palevitz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcus","family":"Fechheimer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milton J.","family":"Cormier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.49.070180.004421"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1985.tb09121.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.105.4.1473"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00397340"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00392436"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1242\/dev.100.2.181","article-title":"Calcium: A regulation system emerges in plant cells","volume":"100","author":"Gilroy S.","year":"1987","journal-title":"Development"},{"key":"e_1_2_1_8_1","first-page":"113","volume-title":"Monoclonal Antibodies: Principles and Practice","author":"Goding J. 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