{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:02:41Z","timestamp":1760644961824},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":5878,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[1989,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The spectrins are a family of cytoskeletal\u2010membrane proteins that have a wide tissue distribution. In the present study, we employed polyclonal antibodies made against mammalian and avian erythroid spectrins as well as mammalian brain spectrin to assess their presence and distributions in the mammalian heart. Western blot analyses revealed that all three antibodies were specific for a 240,000 molecular weight \u03b1\u2010spectrin subunit found in hamster erythrocyte ghost homogenates, whole hamster heart, and isolated hamster cardiac myofibril homogenates. Spectrin staining was absent from the Triton X\u2010100\u2010extracted supernatant fraction of myofibril preparations, suggesting that the protein is linked to the myofibril precipitate after exposure to the detergent. Frozen, unfixed, 2\u2010\u03bcm\u2010thick; sections of adult, Syrian golden hamster cardiac tissue exhibited strong immunofluorescent staining of intercalated discs and Z\u2010bands using all three antibodies. In addition, the mammalian erythroid spectrin antibodies showed staining of the sarcolemma, and in cross section, revealed a delicate internal network of staining that appears to surround individual myofibrils. This may be T\u2010tubule\u2010associated staining. Myofibrils isolated from cardiac myocytes using Triton X\u2010100 show positive Z\u2010band staining using all three antibodies. Double staining with Texas Red\u2010labeled monoclonal desmin and FITC\u2010labeled polyclonal spectrin antibodies revealed that both stained the myofibrillar Z\u2010line regions. These results demonstrate that spectrin is closely associated with the membranes, myofibrils, and intermediate filaments in the mammalian heart.<\/jats:p>","DOI":"10.1002\/cm.970120303","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T03:48:34Z","timestamp":1109216914000},"page":"139-149","source":"Crossref","is-referenced-by-count":21,"title":["Immunocytochemical studies of spectrin in hamster cardiac tissue"],"prefix":"10.1002","volume":"12","author":[{"given":"Dino A.","family":"Messina","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larry F.","family":"Lemanski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.54.070185.001421"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.95.2.478"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970030513"},{"issue":"12","key":"e_1_2_1_5_1","first-page":"7757","article-title":"Brain spectrin","volume":"258","author":"Davis J.","year":"1983","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.44.1.1"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970030531"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1983.244.3.C121"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1986.250.3.C347"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.12.7570"},{"key":"e_1_2_1_11_1","first-page":"300","article-title":"Immunoelectron microscopic localization of \u03b1\u2010actinin and actin in embryonic localization of \u03b1\u2010actinin and actin in embryonic hamster heart cells","volume":"39","author":"Hill C. S.","year":"1986","journal-title":"Eur. J. Cell Biol."},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(83)90080-6"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0735-0651(84)90049-9"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240300202"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970080208"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.159.3811.203"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2828(73)90026-6"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.880070511"},{"key":"e_1_2_1_20_1","first-page":"310","volume-title":"The Biology of Cardiac Myocytes","author":"Messina D. A.","year":"1987"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.16.2.297"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.2.363"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.97.4.1081"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.3.2.269"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(82)90444-5"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.3.802"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.102.6.2088"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.12.1.91"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(88)90237-0"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(79)90732-2"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00713171"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0361-9230(87)90221-8"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(81)90439-5"}],"container-title":["Cell Motility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcm.970120303","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cm.970120303","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T13:37:56Z","timestamp":1697981876000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cm.970120303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,1]]},"references-count":33,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1989,1]]}},"alternative-id":["10.1002\/cm.970120303"],"URL":"https:\/\/doi.org\/10.1002\/cm.970120303","archive":["Portico"],"relation":{},"ISSN":["0886-1544"],"issn-type":[{"value":"0886-1544","type":"print"}],"subject":[],"published":{"date-parts":[[1989,1]]}}}