{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T08:11:41Z","timestamp":1759565501214},"reference-count":65,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":5878,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[1989,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The dynamic changes of the endoplasmic reticulum (ER) in interphase and mitotic cells was detected by the vital fluorescent dye 3,3\u2032\u2010dihexyloxacarbocyanine iodide. Two types of arrays characterize the continuous ER system in the non\u2010muscle PtK2 cell: (1) a lacy network of irregular polygons and (2) long strands of ER that are found aligned along stress fibers. In cross\u2010striated myotubes there was a periodic localization of fluorescence over each I\u2010band corresponding to the positions of the terminal cisternae of the sarcoplasmic reticulum (SR). In contrast to the arrangement in muscle cells, the aligment of the long strands of ER along stress fibers showed no strict periodicity that could be correlated with the sarcomeric units of the stress fibers. The ER and SR arrays seen in living cells were also detected in fixed cells stained with antibodies directed against proteins of the endoplasmic reticulum and sarcoplasmic reticulum, respectively. Observations of vitally stained PtK2 cells at 1 to 2 minute intervals using low light level video cameras and image processing techniques enabled us to see the polygonal ER units form and undergo changes in their shapes. During cell division, the ER, rhodamine 123\u2010stained mitochondria, and phagocytosed fluorescent beads were excluded from the mitotic spindle while soluble proteins were not. No obvious concentration or alignment of membranes could be found associated with the contractile proteins in the cleavage furrow. After completion of cell division there was a redeployment of the ER network in each daughter cell.<\/jats:p>","DOI":"10.1002\/cm.970130408","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T03:34:32Z","timestamp":1109216072000},"page":"301-319","source":"Crossref","is-referenced-by-count":43,"title":["Dynamics of the endoplasmic reticulum in living non\u2010muscle and muscle cells"],"prefix":"10.1002","volume":"13","author":[{"given":"Jean M.","family":"Sanger","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey S.","family":"Dome","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Balraj","family":"Mittal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Avril V.","family":"Somlyo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph W.","family":"Sanger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"406","volume-title":"Molecular Biology of the Cell","author":"Alberts B.","year":"1989"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1139\/b80-100"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1000930104"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4684-5278-5_1"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1242\/jcs.12.1.327","article-title":"Microfilaments and cytoplasmic streaming inhibition of streaming with cytochalasin","volume":"12","author":"Bradley M. 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