{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T06:17:44Z","timestamp":1779344264178,"version":"3.51.4"},"reference-count":59,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":5513,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[1990,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A high molecular weight microtubule binding protein has been isolated from homogenates of <jats:italic>Dictyostelium.<\/jats:italic> Because of its sedimentation velocity (20s), ATP\u2010sensitive binding to microtubules. UV\u2010vanadate\u2010ATP mediated fragmentation, prominent CTPase activity, and its ability to produce limited microtubule movement in vitro, we consider this protein to be a form of cytoplasmic dynein. A polyclonal antibody monospecific to this protein was produced, and dynein's intracellular distribution in ameboid cells was examined by immunofluorescence. The antibody labels a punctate cytoplasmic pattern, localizes to a spherical region adjacent to the nucleus, and also appears to label the nuclei. The punctate staining pattern is consistent with cytoplasmic dynein's proposed function in organelle transport. The spherical juxtanuclear object stained is coincident with this cell's microtubule organizing center, an obvious termination point for minus\u2010end directed microtubule motors. By immunofluorescence, there does not appear to be a substantial amount of dynein in the intranuclear mitotic spindles of <jats:italic>Dictyostelium<\/jats:italic>, These data provide evidence for localization of cytoplasmic dynein in cells, and suggest that <jats:italic>Dictyostelium<\/jats:italic> will be a useful system in which to study the molecular biology of microtubule\u2010associated motor enzymes.<\/jats:p>","DOI":"10.1002\/cm.970150108","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T03:28:01Z","timestamp":1109215681000},"page":"51-62","source":"Crossref","is-referenced-by-count":61,"title":["Identification and immunolocalization of cytoplasmic dynein in <i>dictyostelium<\/i>"],"prefix":"10.1002","volume":"15","author":[{"given":"Michael P.","family":"Koonce","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Richard McIntosh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/295701a0"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_1_4_1","first-page":"169","volume-title":"Cell Movement","author":"Burnside B.","year":"1989"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.103.2.593"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.79.2.573"},{"key":"e_1_2_1_7_1","first-page":"207","article-title":"Immunofluo\u2010rescence of microtubular structures during the cell cycle of Dictyostelium discoideum","volume":"124","author":"Cappuccinelli P.","year":"1981","journal-title":"J. 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