{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T14:46:20Z","timestamp":1758638780831},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":5513,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[1990,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Swiss mouse 3T3 fibroblasts grown on a solid substrate in the presence of 10% serum exhibit cell movement, organelle transport, and cytokinesis. When the serum concentration in the culture medium is decreased to 0.2% for 48 h the serum\u2010deprived cells virtually stop locomoting, spread, decreased organelle transport, and exhibit extensive arrays of stress fibers that are visible with video\u2010enhanced differential interference contrast microscopy and that also incorporate fluorescent analogs of actin and conventional myosin (myosin II). The stress fibers form in a constitutive mannet at the cytoplasm\u2010membrane interface, transport toward the nucleus, and then disappear. The rate of transport of these fibers is quite heterogeneous with average rates in the range of 10\u201320 \u03bcm\/h. When serum\u2010deprived cells are stimulated with mitogens such as 10% serum or 10 nM thrombin, many of the stress fibers immediately begin to shorten, suggesting a contraction. The rate of shortening is approximately two orders of magnitude slower than that of unloaded smooth muscle cells. The fiber shortening is often accompanied by retraction of the edges of the cell and continues for at least the 1st hour post\u2010stimulation.<\/jats:p>","DOI":"10.1002\/cm.970160104","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T03:25:36Z","timestamp":1109215536000},"page":"14-21","source":"Crossref","is-referenced-by-count":52,"title":["Formation, transport, contraction, and disassembly of stress fibers in fibroblasts"],"prefix":"10.1002","volume":"16","author":[{"given":"Kenneth A.","family":"Giuliano","sequence":"first","affiliation":[]},{"given":"D.","family":"Lansing Taylor","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(84)90460-9"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3277283"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.104.4.1019"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041410223"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/294691a0"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"18234","DOI":"10.1016\/S0021-9258(19)84702-6","article-title":"Mitogen\u2010mduced [Ca2+]i changes in individual human fibroblasts","volume":"264","author":"Byron K. L.","year":"1989","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970140410"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.39.1.149"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.107.6.2631"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3576222"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(76)90311-6"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970110403"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.107.4.1505"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/341328a0"},{"key":"e_1_2_1_16_1","volume-title":"Digitized Video Microscopy","author":"Giuliano K. A.","year":"1989"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)84772-5"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.10.3440"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1989.tb08385.x"},{"key":"e_1_2_1_20_1","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1242\/jcs.60.1.331","article-title":"Behaviour and structure of the leading lamella in moving fibroblasts. I. Occurrence and centripetal movement of are\u2010shaped microfilament bundles beneath the dorsal cell surface","volume":"60","author":"Heath J. P.","year":"1983","journal-title":"J. Cell Sci."},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.83.3.633"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.90.1.84"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.107.6.2181"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/243445a0"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00225909"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/338164a0"},{"key":"e_1_2_1_27_1","first-page":"4825","article-title":"Gelsolin\u2010polyphospho\u2010inositide interaction","volume":"264","author":"Janmey P. A.","year":"1989","journal-title":"J. Cell Biol."},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3576221"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(80)90365-7"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240370302"},{"key":"e_1_2_1_31_1","first-page":"1","article-title":"The role of a superficial plasmagel layer in changes of form, locomotion, and division of cells in tissue cultures","volume":"23","author":"Lewis W. H.","year":"1939","journal-title":"Arch. Exp. Zellforsch."},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1050410205"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4613-1915-3_8"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.101.2.372"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.103.6.2747"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970140207"},{"key":"e_1_2_1_37_1","first-page":"217","article-title":"Thrombin as a growth factor: Mechanism of signal transduction","volume":"19","author":"Seuwen K.","year":"1989","journal-title":"Ather. Rev."},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970120106"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3055292"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)61821-5"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.1982.0125"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.86.2.599"},{"key":"e_1_2_1_43_1","first-page":"347","volume-title":"Applications of Fluorescence in the Biomedical Sciences","author":"Taylor D. L.","year":"1986"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.101.2.597"}],"container-title":["Cell Motility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcm.970160104","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cm.970160104","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T14:31:52Z","timestamp":1697985112000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cm.970160104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,1]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1990,1]]}},"alternative-id":["10.1002\/cm.970160104"],"URL":"https:\/\/doi.org\/10.1002\/cm.970160104","archive":["Portico"],"relation":{},"ISSN":["0886-1544"],"issn-type":[{"value":"0886-1544","type":"print"}],"subject":[],"published":{"date-parts":[[1990,1]]}}}