{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T01:13:22Z","timestamp":1770340402669,"version":"3.49.0"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":5148,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[1991,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Microtubules were assembled from purified tubulin in the buffer originally used to study dynamic instability (100 mM PIPES, 2 mM EGTA, 1 mM magnesium, 0.2 mM GTP) and then diluted in the same buffer to study the rate of disassembly. Following a 15\u2010fold dilution, microtubule polymer decreased linearly to about 20% of the starting value in 15 sec. We determined the length distribution of microtubules before dilution, and prepared computer simulations of polymer loss for different assumed rates of disassembly. Our experimental data were consistent with a disassembly rate per microtubules of 60 \u03bcm\/min. This is the total rate of depolymerization for microtubules in the rapid shortening phase, as determined by light microscopy of individual microtubules (Walker et al.: <jats:italic>Journal of Cell Biology<\/jats:italic> 107:1437\u20131448, 1988). We conclude, therefore, that microtubules began rapid shortening at both ends upon dilution. Moreover, since we could detect no lag between dilution and the onset of rapid disassembly, the transition from elongation to rapid shortening apparently occurred within 1 sec following dilution. Assuming that this transition (catastrophe) involves the loss of the GTP cap, and that cap loss is achieved by the sequential dissociation of GTP\u2010tubulin subunits following dilution, we can estimate the maximum size of the cap based on the kinetic data and model interpretation of Walker et al. The cap is probably shorter than 40 and 20 subunits at the plus and minus ends, respectively.<\/jats:p>","DOI":"10.1002\/cm.970180106","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T03:17:22Z","timestamp":1109215042000},"page":"55-62","source":"Crossref","is-referenced-by-count":60,"title":["Dilution\u2010induced disassembly of microtubules: Relation to dynamic instability and the GTP cap"],"prefix":"10.1002","volume":"18","author":[{"given":"William A.","family":"Voter","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. Timothy","family":"O'Brien","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harold P.","family":"Erickson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1242\/jcs.95.1.33","article-title":"Microtubule dynamic instability: numerical simulation of microtubule transition properties using a lateral cap model","volume":"95","author":"Bayley P.","year":"1990","journal-title":"J. Cell Sci."},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"12675","DOI":"10.1016\/S0021-9258(17)38925-1","article-title":"Concerning the anomalous kinetic behavior of microtubules","volume":"260","author":"Caplow M.","year":"1985","journal-title":"J. Biol. 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