{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,29]],"date-time":"2024-08-29T08:28:08Z","timestamp":1724920088676},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":5148,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[1991,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have cloned and sequenced full length cDNAs for <jats:italic>Acanthamoeba<\/jats:italic> profilin\u2010I and profilin\u2010II. The genes and the encoded proteins are nearly identical except for the region between bp 121 and 210 where 35% of the nucleotides and 47% of amino acids differ. Most of these substitutions are conservative, although three of them are responsible for the differences in the isoelectric points of the isoforms [Kaiser et al., Cell Biol., 102:221\u2013226, 1986]. The DNA sequence revealed six corrections in the previously published protein sequence of profilin\u2010I [Ampe et al., J. Biol. Chem. 260:834\u2013840, 1985] and for the first time resolved the ambiguities at the five positions where profilin\u2010IA and \u2010IB differ. The DNA sequence of profilin\u2010II also allowed us to make two corrections in the protein sequence [Ampeet al., FEBS Lett. 228:17\u201321, 1988a]. Probes prepared from the cDNAs revealed 1 profilin\u2010IA gene. one strongly cross\u2010hybridizing profilin\u2010I gene and one strongly reacting profilin\u2010II gene on Southern blots of <jats:italic>Acanthamoeba<\/jats:italic> DNA. Weaker reactions with other genomic DNA fragments leave open the possibility of one additional gene each for profilin\u2010I and profilin\u2010II. Four different profilin RNAs were resolved on Northern blots. It possible to align the sequences of the three <jats:italic>Acanthamoeba<\/jats:italic> profilins with the sequences of nine other profilins from five different phyla. There are only two invariant residues in these profilin sequences, but many pairwise identities and conservative substitutions that indicate considerable divergence of this family of proteins from its ancestral precursor.<\/jats:p>","DOI":"10.1002\/cm.970200209","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T02:56:05Z","timestamp":1109213765000},"page":"169-177","source":"Crossref","is-referenced-by-count":25,"title":["Analysis of cDNA clones for <i>Acanthamoeba<\/i> profilin\u2010I and profilin\u2010II shows end to end homology with vertebrate profilins and a small family of profilin genes"],"prefix":"10.1002","volume":"20","author":[{"given":"Thomas D.","family":"Pollard","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David L.","family":"Rimm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(88)80575-1"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1988.tb13739.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1016\/S0021-9258(20)71174-9","article-title":"The amino acid sequence of Acanthamoeba profilin","volume":"260","author":"Ampe C.","year":"1985","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.1990.9.323"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","unstructured":"Blasco R. Cole N. B. andMoss B.(1991):Sequence analysis expression and deletion of a vaccinia virus gene encoding a homolog of profilin a eukaryotic actin\u2010binding protein. Submitted for publication.","DOI":"10.1128\/jvi.65.9.4598-4608.1991"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(91)90528-E"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(77)90166-8"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(83)90418-9"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2157283"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1848725"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.110.1.105"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.105.2.913"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.102.1.221"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"5910","DOI":"10.1016\/S0021-9258(18)60651-9","article-title":"Human profilin. Molecular cloning, sequence comparison and chromosome analysis","volume":"263","author":"Kwiatkowski D. J.","year":"1988","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.1.12.937"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.8.12.5108"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"13360","DOI":"10.1016\/S0021-9258(18)69312-3","article-title":"Crystallization of Acanthoeba profilin\u2010","volume":"261","author":"Magnus K. A.","year":"1986","journal-title":"I. J. Biol. 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