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Conserved sequences within or between the subclasses have been identified, together with the locations of known epitopes, chemical modifications, and mutations. Evidence is also reviewed concerning the identity of the GTP\u2010binding sites, about which residues are exposed in the assembled microtubule and at subunit:subunit interfaces. These characteristics constrain the possible tertiary structure of the tubulin subunit.<\/jats:p>","DOI":"10.1002\/cm.970200302","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T02:55:16Z","timestamp":1109213716000},"page":"181-189","source":"Crossref","is-referenced-by-count":76,"title":["\u03b1\u2010, \u03b2\u2010, and \u03b3\u2010tubulins: Sequence comparisons and structural constraints"],"prefix":"10.1002","volume":"20","author":[{"given":"Roy G.","family":"Burns","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.14.3.523"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00268a023"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(90)90150-K"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(89)90102-7"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00439a040"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"17141","DOI":"10.1016\/S0021-9258(17)44880-0","article-title":"Binding of dolstain 10 to tubulin at a distinct site for peptide antimitotic agents near the exchangeable nucleotide and vinca alkaloid sites","volume":"265","author":"Bai R.","year":"1990","journal-title":"J. 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