{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:28:17Z","timestamp":1760887697479},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2008,2,24]],"date-time":"2008-02-24T00:00:00Z","timestamp":1203811200000},"content-version":"vor","delay-in-days":5898,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[1992,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A monoclonal antibody was produced, using as antigen a BHK\u201021 cytoskeletal preparation enriched in intermediate filaments (IF) and their associated proteins. This antibody reacted exclusively with a reproducible set of 70\u2010280kD polypeptides present in minor quantities in this preparation, as detected by immunoblot analysis. Based upon several criteria, this immunologically related group of polypeptides was designated as IFAP\u201070\/280kD (IF\u2010Associated Protein): (1) it coisolated with IF in vitro, (2) it co\u2010localized (by both immunofluorescence and immunoelectron microscopy) with IF in situ in all stages of cell spreading, and (3) it segregated in vitro with the 54\/55kD (desmin\/vimentin) structural IF subunit proteins of BHK cells through two cycles of in vitro disassembly\/assembly. Immunogold labeling further localized IFAP\u201070\/280kD to regions of parallel or loosely bundled IF in situ, suggesting a role in regulating the supramolecular organization of IF. When this monoclonal antibody was used for double\u2010label immunofluorescence observations of colchicine\u2010treated BHK cells, it demonstrated the presence of colchicine\u2010sensitive and colchicine\u2010insensitive IF. Anti\u2010IFAP\u201070\/280kD localized entirely to the drug\u2010induced juxtanuclear IF cap, while a polyclonal antibody directed against the desmin\/vimentin structural IF subunits and the previously characterized monoclonal anti\u2010IFAP\u2010300kD [Yang et al., 1985; <jats:italic>J. Cell Biol.<\/jats:italic> 100:620] localized to both the juxtanuclear IF cap and a colchicine\u2010insensitive IF network peripheral to the cap in the same cells. The colchicine\u2010insensitive IF pattern often exhibited similarities to that observed for the actin\u2010based stress fiber system, suggesting that stress fiber association may be an additional factor in IF organization. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cm.970220306","type":"journal-article","created":{"date-parts":[[2005,4,11]],"date-time":"2005-04-11T19:24:15Z","timestamp":1113247455000},"page":"185-199","source":"Crossref","is-referenced-by-count":23,"title":["Colchicine\u2010sensitive and colchicine\u2010insensitive intermediate filament systems distinguished by a new intermediate filament\u2010associated protein, IFAP\u201070\/280kD"],"prefix":"10.1002","volume":"22","author":[{"given":"Hsi\u2010Yuan","family":"Yang","sequence":"first","affiliation":[]},{"given":"Norman","family":"Lieska","sequence":"additional","affiliation":[]},{"given":"Anne E.","family":"Goldman","sequence":"additional","affiliation":[]},{"given":"Robert D.","family":"Goldman","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2008,2,24]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4362.1981.tb00815.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.98.3.847"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.92.3.795"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.1.293"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.4.1117"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1101\/SQB.1982.046.01.018"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/276729a0"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1985.tb50420.x"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.106.3.723"},{"key":"e_1_2_1_11_1","first-page":"177","volume-title":"Cancer Cell, Vol. 1, The Transformed Phenotype","author":"Franke W. 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