{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T06:26:44Z","timestamp":1779344804490,"version":"3.51.4"},"reference-count":63,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4417,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[1993,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Following the discovery of acetylated \u03b1\u2010tubulin in the flagella of <jats:italic>Chlamydomonas<\/jats:italic>, many studies have documented the presence of acetylated \u03b1\u2010tubulin in a variety of evolutionarily divergent organisms. While this posttranslational modification may define an isoform with a unique function, the primary effect of \u03b1\u2010tubulin acetylation remains unknown. To study the function of \u03b1\u2010tubulin acetylation, we have transformed <jats:italic>Chlamydomoas<\/jats:italic>, a organism in which almost all of the flagellar tubulin ad a subset of the cytoplasmic microtubules are acetylated, with a \u03b11\u2010tubulin gene whose product cannot be acetylated. Specifically, the codon for lysine 40, the lysine that is acetylated, has been replaced with the codons of nonacetylatable amino acids. To distinguish mutagenized \u03b1\u2010tubulin from that produced by the two endogenous \u03b1\u2010tubulin genes, mutant \u03b1\u2010tubulin was tagged with an epitope from influenza virus hemagglutinin. Utilizing the constitutive <jats:italic>Chlamydomonas<\/jats:italic> rubisco small subunit S2 promoter, we have obtained in selected clones high levels of nonacetylatable \u03b1\u2010tubulin expression approximating 50\u201370% of the total flagellar \u03b1\u2010tubulin. Immunofluorescence and immunoblot analysis of transformed cells indicated that nonacetylatable \u03b1\u2010tubulin could assemble, along with endogenous \u03b1\u2010tubulin, into both cytoplasmic and flagellar microtubules. However, no gross phenotypic effects were observed, suggesting that the effect of \u03b1\u2010tubulin acetylation is subtle. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cm.970250205","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T02:23:33Z","timestamp":1109211813000},"page":"158-170","source":"Crossref","is-referenced-by-count":119,"title":["High level expression of nonacetylatable \u03b1\u2010tubulin in <i>Chlamydomonas reinhardtii<\/i>"],"prefix":"10.1002","volume":"25","author":[{"given":"Keith G.","family":"Kozminski","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennis R.","family":"Diener","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joel L.","family":"Rosenbaum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00337712"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.113.2.331"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970200302"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.93.1.15"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"9430","DOI":"10.1016\/S0021-9258(19)50441-0","article-title":"Removal of the amino\u2010terminal acidic residues of yeast actin","volume":"267","author":"Cook R. 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