{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T18:30:57Z","timestamp":1761762657829},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4417,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[1993,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have examined the effects of various means of photobleaching on the recovery of fluorescene, movement, and morphology of the microtubules in the neurites of rhodamine\u2010tubulin\u2010injected PC12 cells. We find that, depending on power of and time of exposure to the bleaching beam, we can generate at least three different patterns of fluorescence recovery in regenerating PC12 neurites. If bleaching is performed with a relatively low\u2010power beam for an extended period, fluorescence in polymer recovers very little after 1 hours. Under these conditions, however, tubulin immunostaining is seen extending through the bleach zone, and microtubules are present through the bleached zone in thin section electron micrographs. If bleaching is performed with a high\u2010power laster, for 0.5\u20135 seconds, fluorescence recovery also is quite slow, but electron microscopic observations reveal that no microtubules extend through the bleached region of the neurite, and the uranyl acetate\u2010stained cytoplasm appears more electron lucent than in the unbleached neurite. Finally, if bleaching is performed by very brief exposure to a high\u2010intensity laser beam, resulting in an incomplete reduction of fluorescence intensity through the bleach zone, fluorescence recovery occurs within 20\u201330 minutes, and immunostained microtubules appear intact through the bleach zone; electron microscopy confirms that microtubules extended through the bleached zone of such neurites. In all three cases, movement of the bleach zone is observed in approximately half of the experimental neurites. These results indicate that highly variable microtubule behaviors can be obtained with photobleach technology, presumably due to different levels and pathways of photodamage generated by different bleach protocols. Nevertheless, it is clear that both turnover and movement of microtubules occur in FC12 neurites, and both are likely to be involved in neurite maintenance and growth. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cm.970250405","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T02:22:33Z","timestamp":1109211753000},"page":"345-357","source":"Crossref","is-referenced-by-count":7,"title":["Microtubule behavior in PC12 neurites: Variable results obtained with photobleach technology"],"prefix":"10.1002","volume":"25","author":[{"given":"Charles H.","family":"Keith","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark A.","family":"Farmer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.111.2.495"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490300115"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.86.2.616"},{"key":"e_1_2_1_5_1","first-page":"174a","article-title":"Visualization of compositional domains along individual microtubules (MTs) in cultured neurons: The frayed axon preparation","volume":"115","author":"Brown A.","year":"1991","journal-title":"J. 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