{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T04:11:09Z","timestamp":1760501469385},"reference-count":56,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4052,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous immunolocalization studies using many primate cultured cell lines demonstrated that a microtubule\u2010associated protein of M<jats:sub>r<\/jats:sub> \u223c210,000 which is now called MAP4, is present along the length of microtubules in interphase and mitotic cells [Bulinski and Borisy (1980) J. Cell Biol. 87:802\u2013808; DeBrabander et al. (1981) J. Cell Biol. 91:438\u2013455]. Since MAP4 has been implicated as a microtubule stabilizer, we asked whether all classes of microtubules possess an equal complement of MAP4. We have reexamined the cellular distribution of MAP4, using both conventional double\u2010label immunofluorescence and an antibody blocking technique [Schulze and Kirschner (1987) J. Cell Biol. 104:277\u2013288] to highlight microtubules lacking, or depleted in, MAP4. These techniques have revealed that thin processes extending from monkey kidney cells (TC\u20107), and those made by human neuroblastoma cells (IMR\u201032) in response to retinoic acid, are often deficient in MAP4 immunoreactivity. Since both types of cellular processes contain stable microtubules, which are enriched in detyrosinated (Glu) tubulin, we tested the ability of MAP4 to bind to microtubules made from pure Glu and pure tyrosinated (Tyr) tubulin in vitro. MAP4 bound to both types of microtubules, and the similar saturation level of MAP4 binding to Glu and Tyr microtubules suggested that differential binding to these forms of tubulin does not contribute directly to a mechanism for segregation of MAP4 on microtubules in vivo. In TC\u20107 cells, we also observed MAP4\u2010depletion on single microtubules, distal regions of broad cytoplasmic extensions, and midbodies of dividing cells. MAP4 depletion may reflect recent, rapid growth of microtubules to which MAP4 has not yet bound, or the presence of other MAPs that may compete with MAP4 for binding sites on the MT. We suggest that different levels of MAP4 on microtubules may directly modulate microtubule dynamics within single cells, as well as other microtubule functions such as those involving microtubule motor activity. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cm.970270205","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T02:18:35Z","timestamp":1109211515000},"page":"133-149","source":"Crossref","is-referenced-by-count":36,"title":["Cellular microtubules heterogeneous in their content of microtubule\u2010associated protein 4 (MAP4)"],"prefix":"10.1002","volume":"27","author":[{"given":"Steven J.","family":"Chapin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeannette Chlo\u00eb","family":"Bulinski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.100.2.279"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"5885","DOI":"10.1016\/S0021-9258(18)83632-8","article-title":"A common amino acid sequence in 190\u2010kDa microtubule\u2010associated protein and tau for the promotion of microtubule assembly","volume":"264","author":"Aizawa H.","year":"1989","journal-title":"J. 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