{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T18:44:30Z","timestamp":1772390670240,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4052,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>After the infectious bacterium, <jats:italic>Listeria monocytogenes<\/jats:italic>, is phagocytosed by a host cell, it leaves the lysosome and recruits the host cell's cytoskeletal proteins to assemble a stationary tail composed primarily of actin filaments cross\u2010linked with alpha\u2010actinin. The continual recruitment of contractile proteins to the interface between the bacterium and the tail accompanies the propulsion of the bacterium ahead of the elongating tail. When a bacterium contacts the host cell membrane, it pushes out the membrane into an undulating tubular structure or filopodium that envelops the bacterium at the tip with the tail of cytoskeletal proteins behind it. Previous work has demonstrated that alpha\u2010actinin can be cleaved into two proteolytic fragments whose microinjection into cells interferes with stress fiber integrity. Microinjection of the 53 kD alpha\u2010actinin fragment into cells infected with <jats:italic>Listeria monocytogenes<\/jats:italic>, induces the loss of tails from bacteria and causes the bacteria to become stationary. Infected cells that possess filopodia when injected with the 53 kD fragment lose their filopodia. These results indicate that intact alpha\u2010actinin molecules play an important role in the intracellular motility of <jats:italic>Listeria<\/jats:italic>, presumably by stabilizing the actin fibers in the stationary tails that are required for the bacteria to move forward. Fluorescently labeled vinculin associated with the tails when it was injected into infected cells. Talin antibody staining indicated that this protein, also, is present in the tails. These observations suggest that the tails share properties of attachment plaques normally present in the host cells. This model would explain the ability of the bacterium (1) to move within the cytoplasm and (2) to push out the surface of the cell to form a filopodium. The attachment plaque proteins, alpha\u2010actinin, talin, and vinculin, may bind and stabilize the actin filaments as they polymerize behind the bacteria and additionally could also enable the tails to bind to the cell membrane in the filopodia. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cm.970280202","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T01:58:06Z","timestamp":1109210286000},"page":"97-107","source":"Crossref","is-referenced-by-count":52,"title":["Intact alpha\u2010actinin molecules are needed for both the assembly of actin into the tails and the locomotion of <i>Listeria monocytogenes<\/i> inside infected cells"],"prefix":"10.1002","volume":"28","author":[{"given":"Frederick G.","family":"Dold","sequence":"first","affiliation":[]},{"given":"Jean M.","family":"Sanger","sequence":"additional","affiliation":[]},{"given":"Joseph W.","family":"Sanger","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.10.3867"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.16.6068"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"1068","DOI":"10.1128\/iai.19.3.1068-1075.1978","article-title":"Experimental infection of mouse peritoneal mesothelium with scrub typhus Rickettsia: An ultrastructural study","volume":"19","author":"Ewing E. P.","year":"1978","journal-title":"Infect. Immun."},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90003-H"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1016\/S0021-9258(19)86162-8","article-title":"A rapid purification of alpha\u2010actinin, filamin and a 130,000 dalton protein from smooth muscle","volume":"255","author":"Feramisco J. R.","year":"1980","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/357515a0"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.79.1.268"},{"key":"e_1_2_1_9_1","first-page":"223","article-title":"Rearrangement of myofibrils and intermediate filaments in cultured ventricular cardiomyocytes of the adult rat","volume":"38","author":"Imanaka K.","year":"1988","journal-title":"Mie Med. J."},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"3463","DOI":"10.1128\/iai.59.10.3463-3471.1991","article-title":"Intercellular spread of Shigella flexneri through a monolayer mediated by membranous protrusions and associated with reorganization of the cytoskeleton protein vinculin","volume":"59","author":"Kadurugamuwa J. L.","year":"1991","journal-title":"Infect. Immun."},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.169.3.1291-1297.1987"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90188-I"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.105.3.699"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.11.3477-3486.1990"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(75)90180-4"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1439807"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.116.3.381"},{"key":"e_1_2_1_19_1","first-page":"637","article-title":"Some properties of chicken alpha\u2010actinin","volume":"66","author":"Masaki T.","year":"1969","journal-title":"J. Biochem. (Tokyo)"},{"key":"e_1_2_1_20_1","doi-asserted-by":"crossref","first-page":"4717","DOI":"10.1016\/S0021-9258(18)61254-2","article-title":"Further characterization of a conserved actin\u2010binding 27\u2010kDa fragment of actinogelin and \u03b1\u2010actinins and mapping of their binding sites on the actin molecule by chemical cross\u2010linking","volume":"262","author":"Mimura N.","year":"1987","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.7883\/yoken1952.21.259"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.111.2.721"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1089\/hyb.1990.9.57"},{"key":"e_1_2_1_24_1","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1242\/jcs.94.1.109","article-title":"Identification of a filamin isoform enriched at the ends of stress fibers in chicken fibroblasts","volume":"94","author":"Pavalko M. F.","year":"1989","journal-title":"J. Cell Sci."},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.114.3.481"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1042\/bst0191065"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.167.4.1459"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970280102"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/357442a0"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.99.3.918"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.102.6.2053"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970130408"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.14.5474"},{"key":"e_1_2_1_34_1","first-page":"415A","article-title":"Analysis of intracellular motility and actin polymerization induced by Listeria monocytogenes in PtK2 cells","volume":"111","author":"Sanger J. M.","year":"1990","journal-title":"J. Cell Biol."},{"key":"e_1_2_1_35_1","doi-asserted-by":"crossref","first-page":"3609","DOI":"10.1128\/iai.60.9.3609-3619.1992","article-title":"Host cell actin assembly is necessary and likely to provide the propulsion force for intracellular movement of Listeria monocytogenes","volume":"60","author":"Sanger J. M.","year":"1992","journal-title":"Infect Immun"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8493552"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0923-2508(92)90069-Z"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/357257a0"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.4.1597"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.118.1.71"},{"key":"e_1_2_1_41_1","article-title":"Fluorescence polarization study of actin filaments in Listeria\u2010infected cells","volume":"64","author":"Zhukarev V.","year":"1993","journal-title":"Biophys. J."},{"key":"e_1_2_1_42_1","first-page":"383a","article-title":"Orientation and ordering of actin filament bundles in Listeria\u2010infected cells","volume":"4","author":"Zhukarev V.","year":"1993","journal-title":"Mol. Biol. Cell."}],"container-title":["Cell Motility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcm.970280202","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cm.970280202","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T04:40:01Z","timestamp":1698208801000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cm.970280202"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,1]]},"references-count":41,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,1]]}},"alternative-id":["10.1002\/cm.970280202"],"URL":"https:\/\/doi.org\/10.1002\/cm.970280202","archive":["Portico"],"relation":{},"ISSN":["0886-1544"],"issn-type":[{"value":"0886-1544","type":"print"}],"subject":[],"published":{"date-parts":[[1994,1]]}}}