{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T14:55:46Z","timestamp":1775400946612,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4052,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Listeria monocytogenes<\/jats:italic> can penetrate and multiply within a variety of cell types, including the PtK<jats:sub>2<\/jats:sub> kidney epithelial line. Once released within the cytoplasm, <jats:italic>L. monocytogenes<\/jats:italic> acquires the capacity for rapid movement through the host cell [Dabiri et al., 1990: <jats:italic>Proc. Natl. Acad. Sci.<\/jats:italic> 87:6068\u20106072]. In the process, actin monomers are inserted in proximity to one end of the bacterium, forming a column or tail of actin filaments [Sanger et al., 1992: <jats:italic>Infect. Immun.<\/jats:italic> 60:3609\u20103619]. The rate of new actin filament growth correlates closely with the speed of bacterial migration. In this study we have used fluorescently labeled actin and alpha\u2010actinin to monitor the movement and turnover rate of actin and alpha\u2010actinin molecules in the tails. The half\u2010lives of the actin and alpha\u2010actinin present in the tails are approximately the same: actin, 58.7 sec; alpha\u2010actinin, 55.3 sec. The half\u2010life of alpha\u2010actinin surrounding a dividing bacterium was 30 sec, whereas its half\u2010life in the tails that formed behind the two daughter cells was about 20\u201030% longer. We discovered that the speeds of the bacteria are not constant, but show aperiodic episodes of decreased and increased speeds. There is a fluctuation also in the intensities of the fluorescent probes at the bacterium\/tail interface, implying that there is a fluctuation in the number of actin filaments forming there. There was no strong correlation, however, between these fluctuating intensities and changes in speed of the bacteria. These measurements suggest that while actin polymerization at the bacterial surface is coupled to the movement of the bacterium, the periodic changes in intracellular motility are not a simple function of the number of actin filaments nucleating at the bacterial surfaces. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cm.970280408","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T01:56:36Z","timestamp":1109210196000},"page":"346-358","source":"Crossref","is-referenced-by-count":25,"title":["Dynamics of actin and alpha\u2010actinin in the tails of <i>Listeria monocytogenes<\/i> in Infected PtK<sub>2<\/sub> Cells"],"prefix":"10.1002","volume":"28","author":[{"given":"Dipali","family":"Nanavati","sequence":"first","affiliation":[]},{"given":"Francis T.","family":"Ashton","sequence":"additional","affiliation":[]},{"given":"Jean M.","family":"Sanger","sequence":"additional","affiliation":[]},{"given":"Joseph W.","family":"Sanger","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2164-0947.1951.tb01007.x"},{"key":"e_1_2_1_3_1","first-page":"205","volume-title":"Manual of Clinical Microbiology","author":"Bortolussi R.","year":"1985"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970240402"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.16.6068"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970280202"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1992.tb05252.x"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970260309"},{"key":"e_1_2_1_9_1","article-title":"Effects of cytochalasin and colcemid on cortical flow in coelomocytes","volume":"4","author":"Edds K. 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