{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T23:07:45Z","timestamp":1772752065827,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":4052,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rat basophilic leukemia (RBL\u20102H3) cells undergo morphological and cytoskeletal changes during antigen\u2010induced secretion of allergic mediators. The exact role these changes play in the process of secretion is unclear. Using confocal microscopy we now show that PMA + A23187 causes extensive F\u2010actin rearrangements during secretion of [<jats:sup>3<\/jats:sup>H] 5\u2010HT. We also describe for the first time the association of myosin with F\u2010actin during this secretory process. In unstimulated cells, myosin and F\u2010actin are concentrated at the plasma membrane with no evidence of stress fibres. Upon addition of PMA or A23187, both F\u2010actin and myosin are rearranged into membrane ruffles and discrete aggregations (foci), followed by the formation of parallel stress fibres located on the ventral membrane. This is in contrast to reports in other cell types in which PMA has been described as causing the disruption of F\u2010actin stress fibres. The time course of secretion coincides with the formation of the foci and ruffles whilst the stress fibres form after the majority of secretion has occurred. These changes are accompanied by a 40% decrease in cell height and a two\u2010fold increase in cell spreading and they occur in the absence of extracellular calcium but are inhibited by the protein kinase C inhibitor, Bisindolylmaleimide, which also inhibits secretion. The formation of myosin\u2010decorated stress fibres, foci, and ruffles is not sufficient to cause secretion, as PMA alone induces these changes without any secretion. The relevance of actin and myosin rearrangements for the regulation of secretion is discussed. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cm.970290408","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T01:54:11Z","timestamp":1109210051000},"page":"354-365","source":"Crossref","is-referenced-by-count":28,"title":["PMA and calcium ionophore induce myosin and F\u2010actin rearrangement during histamine secretion from RBL\u20102H3 cells"],"prefix":"10.1002","volume":"29","author":[{"given":"Russell I.","family":"Ludowyke","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuo","family":"Kawasugi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter W.","family":"French","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"2626","DOI":"10.4049\/jimmunol.143.8.2626","article-title":"The rise in concentration of free Ca2+ and of pH provides sequential, synergistic signals for secretion in antigen\u2010stimulated rat basophilic leukemia (RBL\u20102H3) cells","volume":"143","author":"Ali H.","year":"1989","journal-title":"J. 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